Heat shock, visible light or high calcium augment the cytotoxic effects of Ailanthus altissima (Swingle) leaf extracts against Saccharomyces cerevisiae cells

To gain new insight into the antimicrobial potential of Ailanthus altissima Swingle, ethanol leaf extracts were evaluated for the antifungal effects against the model yeast Saccharomyces cerevisae. The extracts inhibited the yeast growth in a dose-dependent manner, and this effect could be augmented...

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Bibliographic Details
Published in:Natural product research 2015-09, Vol.29 (18), p.1744-1747
Main Authors: Popa, Claudia Valentina, Lungu, Liliana, Cristache, Ligia Florentina, Ciuculescu, Crinu, Danet, Andrei Florin, Farcasanu, Ileana Cornelia
Format: Article
Language:English
Subjects:
Ailanthus - chemistry
Ailanthus altissima extracts
anti-fungal synergy
Antifungal Agents - chemistry
calcium
Calcium - chemistry
heat shock
Hot Temperature
Light
Plant Extracts - chemistry
Plant Leaves - chemistry
Saccharomyces cerevisiae
Saccharomyces cerevisiae - drug effects
visible light
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Summary:To gain new insight into the antimicrobial potential of Ailanthus altissima Swingle, ethanol leaf extracts were evaluated for the antifungal effects against the model yeast Saccharomyces cerevisae. The extracts inhibited the yeast growth in a dose-dependent manner, and this effect could be augmented by heat shock, exposure to visible light or exposure to high concentrations of Ca 2+ . Using transgenic yeast cells expressing the Ca 2+ -dependent photoprotein, aequorin, it was found that the leaf extracts induced cytosolic Ca 2+ elevation. Experiments on yeast mutants with defects in Ca 2+ transport demonstrated that the cytotoxicity of the A. altissima leaf extracts (AaLEs) was mediated by transient pulses of Ca 2+ ions which were released into the cytosol predominantly from the vacuole. The investigation of the antifungal synergies involving AaLEs may contribute to the development of optimal and safe combination therapies for the treatment of drug-resistant fungal infections.
ISSN:1478-6419
1478-6427
DOI:10.1080/14786419.2014.998215