Single molecule studies of RNA polymerase II transcription in vitro

Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Elucidating the mechanism by which RNAP II synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. S...

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Bibliographic Details
Published in:Transcription 2014, Vol.5 (1), p.e27608
Main Authors: Horn, Abigail E, Goodrich, James A, Kugel, Jennifer F
Format: Article
Language:English
Subjects:
Fluorescence Resonance Energy Transfer - methods
Gene Expression Regulation
Models, Genetic
Nucleosomes - metabolism
Optical Tweezers
Promoter Regions, Genetic
RNA Polymerase II - chemistry
RNA Polymerase II - metabolism
RNA Polymerase II - physiology
RNA, Messenger - biosynthesis
Transcription, Genetic - physiology
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Summary:Eukaryotic mRNA transcription by RNA polymerase II (RNAP II) is the first step in gene expression and a key determinant of cellular regulation. Elucidating the mechanism by which RNAP II synthesizes RNA is therefore vital to determining how genes are controlled under diverse biological conditions. Significant advances in understanding RNAP II transcription have been achieved using classical biochemical and structural techniques; however, aspects of the transcription mechanism cannot be assessed using these approaches. The application of single-molecule techniques to study RNAP II transcription has provided new insight only obtainable by studying molecules in this complex system one at a time.
ISSN:2154-1272
DOI:10.4161/trns.27608