Characterization of Lewis Antigens in Normal Colon and Gastrointestinal Adenocarcinomas

The Lewis a antigen (Lea)- and Lewis b antigen (Leb)-active glycolipids and glycoproteins in normal colon mucosa, colon carcinoma tumors, and gastrointestinal tumor cell lines were studied by using monoclonal antibodies against Lewis determinants in solid-phase radioimmunoassay, chromatogram binding...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1985-06, Vol.82 (11), p.3552-3556
Main Authors: Blaszczyk, Magdalena, Pak, Koon Yan, Herlyn, Meenhard, Sears, Henry F., Steplewski, Zenon
Format: Article
Language:English
Subjects:
Adenocarcinoma - immunology
Antibodies
Antibodies, Monoclonal
Antigens
Biological and medical sciences
Carcinoma
Cell Line
Cell lines
Chromatography, Thin Layer
Colon
Colon - immunology
Colonic neoplasms
Colonic Neoplasms - immunology
Electrophoresis, Polyacrylamide Gel
Epitopes - analysis
Fundamental and applied biological sciences. Psychology
Fundamental immunology
Gastrointestinal Neoplasms - immunology
Glycolipids
Glycolipids - immunology
Glycoproteins
Glycoproteins - immunology
Humans
Lewis Blood-Group System - immunology
Molecular immunology
Mucosa
Pancreatic Neoplasms - immunology
Radioimmunoassay
Rectal Neoplasms - immunology
Stomach Neoplasms - immunology
Tumor cell line
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Summary:The Lewis a antigen (Lea)- and Lewis b antigen (Leb)-active glycolipids and glycoproteins in normal colon mucosa, colon carcinoma tumors, and gastrointestinal tumor cell lines were studied by using monoclonal antibodies against Lewis determinants in solid-phase radioimmunoassay, chromatogram binding assay, and immunoprecipitation/PAGE. Lea-5 was a major component of the purified non-acid glycolipid fraction from normal colon mucosas from four patients examined and Lea-7 was a minor component in three of these four cases. Leain colon carcinoma of the same patients was expressed as Lea-5 in all four cases and as both Lea-5 and Lea-7 in one of the four cases. All normal colon mucosa samples expressed Leabut not Leb. Leb-6 was expressed in all tumors obtained from the same patients as normal colon mucosa (Lebnegative) samples and in the gastrointestinal tumor cell lines. These findings indicate that Lebis a colon adenocarcinoma-associated antigen and that Leaand Lebare coexpressed in tumor tissue. Evidence is presented for the coexistence of Leaand Lebcarbohydrate determinants in both glycolipid and glycoprotein forms in the same cells.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.82.11.3552