Differential laser-induced perturbation Raman spectroscopy: a comparison with Raman spectroscopy for analysis and classification of amino acids and dipeptides

• Published in: Journal of biomedical optics 2015-04, Vol.20 (4), p.047006-047006
• Main Authors: Oztekin, Erman K, Smith, Sarah E, Hahn, David W
• Format: Article
• Language: English
• Subjects: Algorithms; Amino Acids - analysis; Amino Acids - chemistry; Amino Acids - radiation effects; Dipeptides - analysis; Dipeptides - chemistry; Dipeptides - radiation effects; Lasers; Peptide Mapping - methods; Reproducibility of Results; Sensitivity and Specificity; Spectrum Analysis, Raman - instrumentation; Spectrum Analysis, Raman - methods
• ISSN: 1083-3668; 1560-2281
• DOI: 10.1117/1.JBO.20.4.047006

Differential-laser induced perturbation spectroscopy (DLIPS) is a new spectral analysis technique for classification and identification, with key potential applications for analysis of complex biomolecular systems. DLIPS takes advantage of the complex ultraviolet (UV) laser-material interactions based on difference spectroscopy by coupling low intensity UV laser perturbation with a traditional spectroscopy probe. Here, we quantify the DLIPS performance using a Raman scattering probe in classification of basic constituents of collagenous tissues, namely, the amino acids glycine, l-proline, and l-alanine, and the dipeptides glycine-glycine, glycine-alanine and glycine-proline and compare the performance to a traditional Raman spectroscopy probe via several multivariate analyses. We find that the DLIPS approach yields an ∼40% improvement in discrimination among these tissue building blocks. The effects of the 193-nm perturbation laser are further examined by assessing the photodestruction of targeted material molecular bonds. The DLIPS method with a Raman probe holds promise for future tissue diagnosis, either as a stand-alone technique or as part of an orthogonal biosensing scheme.