Monitoring the ionic content of exhaled breath condensate in various respiratory diseases by capillary electrophoresis with contactless conductivity detection

The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection...

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Bibliographic Details
Published in:Journal of breath research 2015-05, Vol.9 (2), p.027107-027107
Main Authors: Greguš, Michal, Foret, František, Kindlová, Dagmar, Pokojová, Eva, Plutinský, Marek, Doubková, Martina, Merta, Zden k, Binková, Ilona, Sk i ková, Jana, Kubá, Petr
Format: Article
Language:English
Subjects:
Acetates - analysis
Acetates - metabolism
Adult
Aged
Ammonium Compounds - analysis
Ammonium Compounds - metabolism
anions and cations
Asthma - metabolism
Breath Tests - instrumentation
Breath Tests - methods
Butyrates - analysis
Butyrates - metabolism
Calcium - analysis
Calcium - metabolism
Capillary electrophoresis
Chlorides - analysis
Chlorides - metabolism
contactless conductivity detection
Crown Ethers
Cystic Fibrosis - metabolism
Electrophoresis, Capillary
exhaled breath condensate
Female
Humans
Hydrogen-Ion Concentration
Ions
Lactic Acid - analysis
Lactic Acid - metabolism
Magnesium - analysis
Magnesium - metabolism
Male
Middle Aged
Nitrates - analysis
Nitrates - metabolism
Nitrites - analysis
Nitrites - metabolism
Potassium - analysis
Potassium - metabolism
Propionates - analysis
Propionates - metabolism
Pulmonary Disease, Chronic Obstructive - metabolism
Pulmonary Fibrosis - metabolism
Respiratory diseases
Sarcoidosis, Pulmonary - metabolism
Sodium - analysis
Sodium - metabolism
Sulfates - analysis
Sulfates - metabolism
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Summary:The analysis of an ionic profile of exhaled breath condensate (EBC) by capillary electrophoresis with contactless conductivity detection and double opposite end injection, is demonstrated. A miniature sampler made from a 2 ml syringe and an aluminium cooling cylinder was used for the fast collection of EBC (under one minute). Analysis of the collected EBC was performed in a 60 mM 2-(N-morpholino)ethanesulfonic acid, 60 mM L-histidine background electrolyte with 30 µM cetyltrimethylammonium bromide and 2 mM 18-crown-6 at pH 6, and excellent repeatability of migration times (RSD  
ISSN:1752-7155
1752-7163
1752-7163
DOI:10.1088/1752-7155/9/2/027107