Renalase Protects the Cardiomyocytes of Sprague-Dawley Rats Against Ischemia and Reperfusion Injury by Reducing Myocardial Cell Necrosis and Apoptosis

Background/Aims: Renalase, a novel flavoprotein expressed in the kidney and heart, reduces renal tubular necrosis and apoptosis, which suggests that it might protect against necrosis and/or apoptosis in myocardial ischemia reperfusion injury (MIRI). The present study thus explored the effects of ren...

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Bibliographic Details
Published in:Kidney & blood pressure research 2015-01, Vol.40 (3), p.215-222
Main Authors: Li, Xiaogang, Xie, Zijian, Lin, Minyuan, Huang, Ruixia, Liang, Zhongshu, Huang, Wei, Jiang, Weihong
Format: Article
Language:English
Subjects:
Animals
Apoptosis - drug effects
Blood Pressure - drug effects
Gene Expression Regulation, Enzymologic
Genetic Vectors - administration & dosage
Heart Rate - drug effects
Injections
Ischemia and reperfusion injury
Lentivirus
Lentivirus - genetics
Male
Monoamine Oxidase - genetics
Myocardial Reperfusion Injury - pathology
Myocardial Reperfusion Injury - prevention & control
Myocytes, Cardiac - drug effects
Myocytes, Cardiac - pathology
Necrosis
Original Paper
Pericardium
Rats
Rats, Sprague-Dawley
Recombinant Proteins - therapeutic use
Recombinant renalase protein
Renalase
RNA Interference
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Summary:Background/Aims: Renalase, a novel flavoprotein expressed in the kidney and heart, reduces renal tubular necrosis and apoptosis, which suggests that it might protect against necrosis and/or apoptosis in myocardial ischemia reperfusion injury (MIRI). The present study thus explored the effects of renalase on Sprague-Dawley (SD) rats subjected to MIRI. Methods: We used Lentivirus-mediated RNA interference (RNAi) to inhibit the renalase gene expression in the heart tissue via pericardial cavity injection. The MIRI animal modal was established by blocking the left anterior descending artery for 45mins followed by 4h of reperfusion. Real-time PCR and western blotting were used to detect renalase expression in the heart tissue. Double staining and TUNEL were used to detect the necrosis and apoptosis in the myocardial cells, respectively. Results: All rats subjected to MIRI exhibited lower levels of renalase in the heart tissue than did the sham-operated group (P
ISSN:1420-4096
1423-0143
DOI:10.1159/000368497