Effect of systemic clarithromycin and prednisolone on histamine-induced otitis media in guinea pigs

• Published in: Acta oto-laryngologica 2015-10, Vol.135 (10), p.978-984
• Main Authors: Kozan, Günay, Aktan, Bülent, Sakat, Muhammed Sedat, Kurt, Sezgin, Öner, Fatih, Kara, Adem
• Format: Article
• Language: English
• Subjects: Animals; Anti-Bacterial Agents - administration & dosage; antioxidant; clarithromycin; Clarithromycin - administration & dosage; cytokines; Cytokines - metabolism; Disease Models, Animal; Dose-Response Relationship, Drug; Drug Therapy, Combination; Ear, Middle - drug effects; Ear, Middle - pathology; Glucocorticoids - administration & dosage; Guinea Pigs; Histamine - toxicity; Injections, Intraperitoneal; Male; Otitis media with effusion; Otitis Media with Effusion - chemically induced; Otitis Media with Effusion - diagnosis; Otitis Media with Effusion - drug therapy; prednisolone; Prednisolone - administration & dosage
• ISSN: 0001-6489; 1651-2251
• DOI: 10.3109/00016489.2015.1058526

Conclusion: The results of the study showed that clarithromycin has anti-inflammatory and antioxidant effects and, when it is combined with prednisolone, those effects gain strength. Objectives: The present study aims to investigate the effects that the antioxidant and anti-inflammatory activities of clarithromycin and/or prednisolone have on experimental otitis media in effusion-induced guinea-pigs. Method and results: In this study, 35-male guinea pigs were randomly divided into five-groups. For the experimental otitis media, intra-tympanic histamine (0.1 ml) was injected into the guinea pigs in all of the groups except the control group. Then, 24-h after the intra-tympanic injections, clarithromycin (15 mg/kg/day) and/or prednisolone (1 mg/kg/day) were applied intraperitoneally to the guinea-pigs for 7-days. The biochemical analysis showed an increase in antioxidant capacity and a decrease in oxidant status and malondialdehyde (MDA) levels in the clarithromycin group and the prednisolone group and especially in the clarithromycin+prednisolone group, as compared to the experimental group (p < 0.05). In the cytokine analysis, lower levels of interleukin (IL)-6 and IL-17A and higher IL-10 were found in the clarithromycin, prednisolone, and clarithromycin+prednisolone groups than in the experimental group (p < 0.05). Furthermore, the histologic analyses showed histopathologic changes in the middle ear mucosa of the experimental group, but comparatively fewer-histopathologic changes were observed in the clarithromycin, prednisolone, and clarithromycin+prednisolone groups.