Design and evaluation of mucoadhesive microemulsion for neuroprotective effect of ibuprofen following intranasal route in the MPTP mice model

Background: The present study is to investigate the neuroprotective effect of ibuprofen by intranasal administration of mucoadhesive microemulsion (MMEI) against inflammation-mediated by dopaminergic neurodegeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's d...

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Published in:Drug development and industrial pharmacy 2016-08, Vol.42 (8), p.1340-1350
Main Authors: Mandal, Surjyanarayan, Mandal, Snigdha Das, Chuttani, Krishna, Sawant, Krutika K, Subudhi, Bharat Bhushan
Format: Article
Language:English
Subjects:
1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine - pharmacology
Adhesives - chemistry
Adhesives - pharmacology
Administration, Intranasal - methods
Animals
Box-Behnken design
Capmul MCM
Corpus Striatum - drug effects
Corpus Striatum - metabolism
Disease Models, Animal
Dopamine - metabolism
Emulsions - chemistry
Emulsions - pharmacology
flux
Ibuprofen - chemistry
Ibuprofen - pharmacology
Inflammation - chemically induced
Inflammation - drug therapy
Male
male C57BL/6 mice
Mice
Mice, Inbred C57BL
Neurons - drug effects
Neurons - metabolism
Neuroprotective Agents - chemistry
Neuroprotective Agents - pharmacology
Parkinson Disease - drug therapy
response surface methodology (RSM)
Substantia Nigra - drug effects
Substantia Nigra - metabolism
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Summary:Background: The present study is to investigate the neuroprotective effect of ibuprofen by intranasal administration of mucoadhesive microemulsion (MMEI) against inflammation-mediated by dopaminergic neurodegeneration in 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) model of Parkinson's disease (PD). Methods: Ibuprofen-loaded polycarbophil-based MMEI was developed by using response surface methodology (RSM). Ibuprofen with dose of 2.86 mg/kg/day was administered intranasally to male C57BL/6 mice for two consecutive weeks which were pre-treated with four intraperitoneal injections of MPTP (20 mg/kg of body weight) at 2 h intervals. Immunohistochemistry was performed. Results: Optimal MMEI was stable and non-ciliotoxic with 66.29 ± 4.15 nm as average globule size and −20.9 ± 3.98 mV as zeta potential. PDI value and transmission electron microscopy result showed the narrow globule size distribution of MMEI. The result showed that all three independent variables had a significant effect (p 
ISSN:0363-9045
1520-5762
DOI:10.3109/03639045.2015.1135936