Levels of Schistosoma mansoni Circulating Antigen in Chronic Hepatitis C Patients with Different Stages of Liver Fibrosis

• Published in: Journal of immunoassay & immunochemistry 2016-05, Vol.37 (3), p.316-330
• Main Authors: Attallah, Abdelfattah M., El-Far, Mohamed, Omran, Mohamed M., Farid, Khaled, Attallah, Ahmed A., Abd-Elaziz, Dalal, El-Bendary, Mohamed S., El-Dosoky, Ibrahim, Ismail, Hisham
• Format: Article
• Language: English
• Subjects: Adult; Animals; Antibodies, Monoclonal - immunology; antigen detection; Antigen-Antibody Reactions; Antigens, Helminth - blood; Antigens, Helminth - immunology; Blotting, Western; Egypt; Enzyme-Linked Immunosorbent Assay; Female; Hepatitis C virus; Hepatitis C, Chronic - blood; Hepatitis C, Chronic - immunology; Humans; interferon-γ; Liver Cirrhosis - blood; Liver Cirrhosis - immunology; Liver Cirrhosis - parasitology; liver fibrosis; Male; Middle Aged; schistosoma; Schistosoma mansoni; Schistosoma mansoni - immunology; Schistosoma mansoni - isolation & purification; Schistosomiasis mansoni - blood; Schistosomiasis mansoni - immunology; Schistosomiasis mansoni - parasitology; Severity of Illness Index; Young Adult
• ISSN: 1532-1819; 1532-4230
• DOI: 10.1080/15321819.2015.1135163

The goal of this study was to determine the levels of S. mansoni antigen in different liver fibrosis stages with chronic hepatitis C (CHC) Egyptian patients. A total of 174 CHC patients showing HCV-NS4 antigen and HCV- RNA in their sera were included. S. mansoni antigen was detected in serum using Western blot and ELISA. The levels of interferon-γ (IFN- γ) were determined using ELISA. The 50 kDa S. mansoni antigen discriminated patients infected with S. mansoni from healthy individuals with 0.93 area under curve (AUC), 92% sensitivity, and 97% specificity. The level of S. mansoni antigen (μg/ml) was significantly (P < 0.0001) increased with the progression of liver fibrosis stages (26.9 ± 17.5 in F1, 42.1 ± 25.2 in F2, 49.8 ± 30.3 in F3 and 62.2 ± 26.3 μg/mL in F4 liver cirrhosis), 26.9 ± 17.59 in significant fibrosis (F2-F4); 51.2 ± 27.9 in advanced fibrosis (F3-F4). A significant correlation (r = 0.506; P < 0.0001) was shown between the levels of the S. mansoni antigen and the HCV-NS4 antigen. In conclusion, the presence of S. mansoni antigen in different liver fibrosis stages of CHC patients confirming that concomitant schistosome infection aggravates liver disease.