Phytoremediation of the organic Xenobiotic simazine by p450-1a2 transgenic Arabidopsis thaliana plants

• Published in: International journal of phytoremediation 2016-07, Vol.18 (7), p.738-746
• Main Authors: Azab, Ehab, Ahmad K. Hegazy, Mohamed E. El-Sharnouby, Hassan E. Abd Elsalam
• Format: Article
• Language: English
• Subjects: Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis thaliana; Biodegradation, Environmental; Bioremediation; culture media; Cytochrome P-450 CYP1A2 - genetics; Cytochrome P-450 CYP1A2 - metabolism; Genes; Genotype & phenotype; greenhouse experimentation; Herbicides; Herbicides - metabolism; human CYP1A2; Humans; insecticides; isozymes; Markers; Phytoremediation; Plant growth; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; polluted soils; Roots; simazine; Simazine - metabolism; Soils; Transformations; Transgenic; Transgenic plants; western blot analysis
• ISSN: 1549-7879; 1522-6514; 1549-7879
• DOI: 10.1080/15226514.2015.1133559

The potential use of human P450-transgenic plants for phytoremediation of pesticide contaminated soils was tested in laboratory and greenhouse experiments. The transgenic P450 CYP1A2 gene Arabidopsis thaliana plants metabolize number of herbicides, insecticides and industrial chemicals. The P450 isozymes CYP1A2 expressed in A. thaliana were examined regarding the herbicide simazine (SIM). Transgenic A. thaliana plants expressing CYP1A2 gene showed significant resistance to SIM supplemented either in plant growth medium or sprayed on foliar parts. The results showed that SIM produces harmful effect on both rosette diameter and primary root length of the wild type (WT) plants. In transgenic A. thaliana lines, the rosette diameter and primary root length were not affected by SIM concentrations used in this experiment. The results indicate that CYP1A2 can be used as a selectable marker for plant transformation, allowing efficient selection of transgenic lines in growth medium and/or in soil-grown plants. The transgenic A. thaliana plants exhibited a healthy growth using doses of up to 250 μmol SIM treatments, while the non-transgenic A. thaliana plants were severely damaged with doses above 50 μmol SIM treatments. The transgenic A. thaliana plants can be used as phytoremediator of environmental SIM contaminants.