Structure-activity correlation in transfection promoted by pyridinium cationic lipids

The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture - clog  P , lipid length, p...

Full description

Saved in:
Bibliographic Details
Published in:Organic & biomolecular chemistry 2016-01, Vol.14 (11), p.38-39
Main Authors: Parvizi-Bahktar, P, Mendez-Campos, J, Raju, L, Khalique, N. A, Jubeli, E, Larsen, H, Nicholson, D, Pungente, M. D, Fyles, T. M
Format: Article
Language:English
Subjects:
Animals
Cations - chemistry
Cations - toxicity
Cell Survival - drug effects
CHO Cells
Cricetulus
DNA - administration & dosage
DNA - genetics
Galactosidases - genetics
Lipids - chemistry
Lipids - toxicity
Plasmids - administration & dosage
Plasmids - genetics
Pyridinium Compounds - chemistry
Pyridinium Compounds - toxicity
Transfection - methods
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The efficiency of the transfection of a plasmid DNA encoding a galactosidase promoted by a series of pyridinium lipids in mixtures with other cationic lipids and neutral lipids was assessed in CHO-K1 cells. We identify key molecular parameters of the lipids in the mixture - clog  P , lipid length, partial molar volume - to predict the morphology of the lipid-DNA lipoplex and then correlate these same parameters with transfection efficiency in an in vitro assay. We define a Transfection Index that provides a linear correlation with normalized transfection efficiency over a series of 90 different lipoplex compositions. We also explore the influence of the same set of molecular parameters on the cytotoxicity of the formulations. The efficiency of transfection of a plasmid DNA promoted by a series of pyridinium lipids is correlated with molecular parameters of the lipids.
ISSN:1477-0520
1477-0539
DOI:10.1039/c6ob00041j