Hyphal tip cytoplasmic organization in four zygomycetous fungi

We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitu...

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Bibliographic Details
Published in:Mycologia 2016-05, Vol.108 (3), p.533-542
Main Authors: Fisher, Karen E., Roberson, Robert W.
Format: Article
Language:English
Subjects:
apical vesicle crescent
CELL BIOLOGY/ULTRASTRUCTURE
Coemansia
cryofixation
Cytoplasm - ultrastructure
electron microscopy
Fungi - growth & development
Fungi - ultrastructure
Hyphae - growth & development
Hyphae - ultrastructure
hyphal growth
light microscopy
Microscopy, Electron, Transmission
Mortierella
Mucor
phylogeny
vesicle organization
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Summary:We have examined the hyphal tip structure in four zygomycetous fungi: Mortierella verticillata (Mortierellales), Coemansia reversa (Kickxellales), Mucor indicus and Gilbertella persicaria (Mucorales) using both light and transmission electron microscopy. We have used cryofixation and freeze-substitution methods to preserve fungal hyphae for transmission electron microscopy, which yielded improved preservation of ultrastructural details. Our research has confirmed studies that described the accumulation of secretory vesicles as a crescent at the hyphal apex (i.e. the apical vesicle crescent [AVC]) and provided a more detailed understanding of the vesicle populations. In addition, we have been able to observe the behavior of the AVC during hyphal growth in M. indicus and G. persicaria.
ISSN:0027-5514
1557-2536
DOI:10.3852/15-226