Transfer of Codominant Markers by Isolated Metaphase Chromosomes in Chinese Hamster Ovary Cells

Codominant mutations to methotrexate and ouabain resistance in Chinese hamster ovary cells can be transferred to recipient Chinese hamster ovary cells by isolated metaphase chromosomes. For methotrexate, both the structural change and the increased activity of dihydrofolate reductase (5,6,7,8-tetrah...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1977-08, Vol.74 (8), p.3480-3484
Main Authors: Spandidos, Demetrios A., Siminovitch, Louis
Format: Article
Language:English
Subjects:
Cell Cycle - drug effects
Cell Line
Cell lines
CHO cells
Chromosomes
Chromosomes - drug effects
Chromosomes - physiology
Drug Resistance
Enzymes
Genes
Genes, Dominant
Genetic mutation
Metaphase
Metaphase - drug effects
Methotrexate - pharmacology
Mutation
Ouabain - pharmacology
Ovaries
Phenotypes
Somatic cells
Tetrahydrofolate Dehydrogenase - genetics
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Summary:Codominant mutations to methotrexate and ouabain resistance in Chinese hamster ovary cells can be transferred to recipient Chinese hamster ovary cells by isolated metaphase chromosomes. For methotrexate, both the structural change and the increased activity of dihydrofolate reductase (5,6,7,8-tetrahydrofolate:NADP+oxidoreductase EC 1.5.1.3), characteristic of the donor cells, are observed in the transferents (cells that carry and express functions derived by chromosome transfer). The transferents are unstable in the absence of selection although stable clones can be isolated. From results obtained by fractionation of chromosomes and transfer to recipients, the methotrexate and ouabain markers can be assigned to the middle and large size-classes of chromosomes, respectively. By fractionation and transfer of chromosomes, from transferents to new recipients, evidence has been obtained that chromosome integration is not restricted to a particular chromosomal site in the recipient.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.74.8.3480