Single-molecule imaging reveals the stoichiometry change of β2-adrenergic receptors by a pharmacological biased ligand

The stoichiometry of the β2-adrenergic receptor (β2AR) was determined using single-molecule fluorescence imaging in living cells. The results showed that β2AR mainly existed as monomers under physiological conditions and exhibited β-arrestin-dependent dimerization upon stimulation with the pharmacol...

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Bibliographic Details
Published in:Chemical communications (Cambridge, England) England), 2016-06, Vol.52 (44), p.7086
Main Authors: Sun, Yahong, Li, Nan, Zhang, Mingliang, Zhou, Wei, Yuan, Jinghe, Zhao, Rong, Wu, Jimin, Li, Zijian, Zhang, Youyi, Fang, Xiaohong
Format: Article
Language:English
Online Access:Get full text
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Summary:The stoichiometry of the β2-adrenergic receptor (β2AR) was determined using single-molecule fluorescence imaging in living cells. The results showed that β2AR mainly existed as monomers under physiological conditions and exhibited β-arrestin-dependent dimerization upon stimulation with the pharmacological biased ligand carvedilol. The association of β2AR dimerization with biased signalling is revealed.
ISSN:1364-548X
DOI:10.1039/c6cc00628k