Facile synthesis of Cu(2+)-modified mesoporous silica-coated magnetic graphene composite for enrichment of microcystin-LR followed by mass spectrometry analysis

• Published in: Talanta (Oxford) 2016-07, Vol.154, p.183
• Main Authors: Liu, Shasha, Deng, Chunhui, Zhang, Xiangmin
• Format: Article
• Language: English
• Subjects: Animals; Graphite; Humans; Magnetics; Mass Spectrometry; Microcystins; Porosity; Silicon Dioxide
• ISSN: 1873-3573
• DOI: 10.1016/j.talanta.2016.03.064

MCs is a group of potent hepatotoxic peptides produced by cyanobacterial in eutrophic water, among which microcystin-LR is the most abundant and toxic. Long-time accumulation of even trace dosage from drinking water would cause significantly hepatic injury to animal and humans. Here we reported a novel Cu(2+)-modified mesoporous silica coated magnetic graphene composite (magG@mSiO2@-Cu(2+)) through mild sol-gel process and surface modification. Next, the composites were successfully applied for enrichment and separation of microcystin-LR followed by MALDI-TOF MS analysis based on the virtues of excellent hydrophilicity, high surface area (261cm(2)g(-1)), sensitively magnetic separation property, accessible porosity (3.10nm) and large amount of modified Cu(2+) ions. Even performed in a lower concentration (0.5μg/L), at which microcystin-LR could not be detected directly, after treatment with the composites the S/N ratio could appear to be 82.93. Furthermore, the novel composites also exhibited high enrichment efficiency in real water sample. It provided a sensitive and efficient technique for enrichment and detection of microcystin-LR and developed a potent method for separation of pollutant in contaminated water.