Evaluating long-term cellular effects of the arsenic species thio-DMA(V): qPCR-based gene expression as screening tool

• Published in: Journal of trace elements in medicine and biology 2016-09, Vol.37, p.78
• Main Authors: Ebert, Franziska, Thomann, Marlies, Witt, Barbara, Müller, Sandra M, Meyer, Sören, Weber, Till, Christmann, Markus, Schwerdtle, Tanja
• Format: Article
• Language: English
• Subjects: Cacodylic Acid - analogs & derivatives; Cacodylic Acid - toxicity; Cell Death - drug effects; Cell Death - genetics; DNA Damage - drug effects; DNA Damage - genetics; DNA Repair - drug effects; DNA Repair - genetics; Gene Expression Profiling; Humans; Mitochondrial Membranes - drug effects; Real-Time Polymerase Chain Reaction
• ISSN: 1878-3252
• DOI: 10.1016/j.jtemb.2016.06.004

Thio-dimethylarsinic acid (thio-DMA(V)) is a human urinary metabolite of the class 1 human carcinogen inorganic arsenic as well as of arsenosugars. Thio-DMA(V) exerts strong cellular toxicity, whereas its toxic modes of action are not fully understood. For the first time, this study characterises the impact of a long-term (21days) in vitro incubation of thio-DMA(V) on the expression of selected genes related to cell death, stress response, epigenetics and DNA repair. The observed upregulation of DNMT1 might be a cellular compensation to counterregulate the in a very recent study observed massive global DNA hypomethylation after chronic thio-DMA(V) incubation. Moreover, our data suggest that chronic exposure towards subcytotoxic, pico- to nanomolar concentrations of thio-DMA(V) causes a stress response in human urothelial cells. The upregulation of genes encoding for proteins of DNA repair (Apex1, Lig1, XRCC1, DDB2, XPG, ATR) as well as damage response (GADD45A, GADD45G, Trp53) indicate a potential genotoxic risk emanating from thio-DMA(V) after long-term incubation.