Characterization and in vitro evaluation of nimotuzumab conjugated with cisplatin-loaded liposomes

In this paper, we report the conjugation of the humanized monoclonal antibody nimotuzumab with cisplatin-loaded liposomes and the in vitro evaluation of its affinity for tumor cells. The conjugation procedure was performed through derivatization of nimotuzumab with N-succinimidyl S-acetylthioacetate...

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Bibliographic Details
Published in:Journal of liposome research 2017-10, Vol.27 (4), p.274-282
Main Authors: Vázquez-Becerra, Héctor, Pérez-Cárdenas, Enrique, Muñiz-Hernández, Saé, Izquierdo-Sánchez, Vanessa, Medina, Luis Alberto
Format: Article
Language:English
Subjects:
Antibodies, Monoclonal, Humanized - chemistry
Antibodies, Monoclonal, Humanized - metabolism
Antineoplastic Agents - chemistry
Antineoplastic Agents - immunology
Antineoplastic Agents - pharmacology
Carcinoma, Squamous Cell - metabolism
Cell Line, Tumor
Cisplatin - administration & dosage
Cisplatin - chemistry
Drug carriers
Drug Carriers - chemistry
Drug Liberation
Drug Stability
EGFR
Green Fluorescent Proteins - immunology
Humans
immunoliposomes
Liposomes - chemical synthesis
Lung - metabolism
Lung Neoplasms - metabolism
Maleimides - chemistry
monoclonal antibody
Particle Size
Phosphatidylethanolamines - chemistry
Polyethylene Glycols - chemistry
Succinimides - chemistry
Sulfides - chemistry
Surface Properties
tumor cell targeting
Tumor Cells, Cultured
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Summary:In this paper, we report the conjugation of the humanized monoclonal antibody nimotuzumab with cisplatin-loaded liposomes and the in vitro evaluation of its affinity for tumor cells. The conjugation procedure was performed through derivatization of nimotuzumab with N-succinimidyl S-acetylthioacetate (SATA) followed by a covalent attachment with maleimide groups at the end of PEG-DSPE chains located at the membrane of pre-formed liposomes. Confocal microscopy was performed to evaluate the immunoliposome affinity for EGFR antigens from human epidermoid carcinoma (A-431) and normal lung (MRC-5) cell lines. Results showed that the procedures implemented in this work do not affect the capability of the nimotuzumab-immunoliposomes to recognize the tumor cells, which overexpress the EGFR antigens.
ISSN:0898-2104
1532-2394
DOI:10.1080/08982104.2016.1207665