Globulin and albumin-2 associated with protein bodies in Amaranthus cruentus seeds

To examine whether albumin-2, a specific protein found only in amaranth seeds so far, is associated with protein bodies, we isolated protein bodies from Amaranthus cruentus seed embryos by rate-zonal centrifugation with a sucrose gradient. Most protein bodies in the final preparation were intact whe...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1998-01, Vol.62 (6), p.1231-1233
Main Authors: Nakamura, R. (Osaka City Univ. (Japan)), Konishi, Y, Kojima, A, Nakatani, N
Format: Article
Language:English
Subjects:
Agronomy. Soil science and plant productions
albumin-2
ALBUMINAS
ALBUMINE
ALBUMINS
AMARANTHUS CRUENTUS
Amaranthus cruentus L
Biological and medical sciences
Chemical constitution
Economic plant physiology
Fundamental and applied biological sciences. Psychology
globulin
GLOBULINAS
GLOBULINE
GLOBULINS
GRAINE
Plant physiology and development
protein body
SEEDS
SEMILLA
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Summary:To examine whether albumin-2, a specific protein found only in amaranth seeds so far, is associated with protein bodies, we isolated protein bodies from Amaranthus cruentus seed embryos by rate-zonal centrifugation with a sucrose gradient. Most protein bodies in the final preparation were intact when observed by electron microscopy. Profiles by SDS-PAGE showed that the isolated protein bodies contained globulin and albumin-2
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.62.1231