Improvement of peroxyl radical scavenging bioassay based on bactericidal action of tert-butyl peroxyl radical

An improved bioassay for measurement of peroxyl radical scavenging aCtivity is described. The modifications included the use of (1) Enterococcus faecium strain S, (2) 25% dimethyl sulfoxide, (3) L-ascorbic acid for the termination of the bactericidal reaction, and (4) the concentration of antioxidan...

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Bibliographic Details
Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 1999-01, Vol.63 (3), p.577-580, Article 577
Main Authors: Kawasumi, T. (Japan Women's Univ., Tokyo), Sato, M, Tsuriya, Y, Ueno, S
Format: Article
Language:English
Subjects:
antioxidant
ANTIOXIDANTES
ANTIOXIDANTS
ANTIOXYDANT
bioassay
BIOASSAYS
Biological and medical sciences
ENSAYO BIOLOGICO
General and cellular metabolism. Vitamins
Medical sciences
Pharmacology. Drug treatments
SINERGISMO
SYNERGISM
SYNERGISME
t-butyl peroxyl radical scavenging
TEST BIOLOGIQUE
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Summary:An improved bioassay for measurement of peroxyl radical scavenging aCtivity is described. The modifications included the use of (1) Enterococcus faecium strain S, (2) 25% dimethyl sulfoxide, (3) L-ascorbic acid for the termination of the bactericidal reaction, and (4) the concentration of antioxidants that gives 75% of the log (colony forming unit/ml) of the control reaction (without methemoglobin or tert-butyl hydroperoxide), for the expression of antioxidant activity
ISSN:0916-8451
1347-6947
DOI:10.1271/bbb.63.577