CB5C affects the glucosinolate profile in Arabidopsis thaliana

• Published in: Plant signaling & behavior 2016-08, Vol.11 (8), p.e1160189-e1160189
• Main Authors: Vik, Daniel, Crocoll, Christoph, Andersen, Tonni Grube, Burow, Meike, Halkier, Barbara Ann
• Format: Article
• Language: English
• Subjects: Acetates - pharmacology; alleles; Arabidopsis; Arabidopsis - drug effects; Arabidopsis - genetics; Arabidopsis - metabolism; Arabidopsis Proteins - genetics; Arabidopsis Proteins - metabolism; Arabidopsis thaliana; biomass; biosynthesis; Cyclopentanes - pharmacology; cytochrome b5; cytochrome P-450; Cytochrome P-450 Enzyme System - genetics; Cytochrome P-450 Enzyme System - metabolism; cytochrome P450; Cytochromes b5 - genetics; Cytochromes b5 - metabolism; DNA, Bacterial - genetics; Gene Expression Regulation, Plant - drug effects; Gene Expression Regulation, Plant - genetics; genetic lines; glucosinolates; Glucosinolates - metabolism; jasmonate; mammals; mechanism of action; methyl jasmonate; mutants; Oxylipins - pharmacology; phenotype; Plants, Genetically Modified - drug effects; Plants, Genetically Modified - genetics; Plants, Genetically Modified - metabolism; Research Paper; specialized metabolism; transfer DNA
• ISSN: 1559-2316; 1559-2324; 1559-2324
• DOI: 10.1080/15592324.2016.1160189

Cytochrome b 5 (CB5) proteins are small heme-binding proteins, that influence cytochrome P450 activity. While only one CB5 isoform is found in mammals, higher plants have several isoforms of these proteins. The roles of the many CB5 isoforms in plants remain unknown. We hypothesized that CB5 proteins support the cytochrome P450 enzymes of plant specialized metabolism and found CB5C from Arabidopsis thaliana to co-express with glucosinolate biosynthetic genes. We characterized the glucosinolate profiles of 2 T-DNA insertion mutants of CB5C, and found that long-chained aliphatic glucosinolates were reduced in one of the mutant lines - a phenotype that was exaggerated upon methyl-jasmonate treatment. These results support the hypothesis, that CB5C influences glucosinolate biosynthesis, however, the mode of action remains unknown. Furthermore, the mutants differed in their biomass response to methyl jasmonate treatment. Thereby, our results highlight the varying effects of T-DNA insertion sites, as the 2 analyzed alleles show different phenotypes.