Culture time and reagent minimization in the chemical PCC assay

The possibility to reduce the culture time and volume of blood and reagents required for the chemical Premature Chromosome Condensation (PCC) assay is demonstrated in this work. Peripheral whole blood was exposed to gamma radiation (1-20 Gy). Lymphocytes were cultured for 40 h, using 50 μl of blood...

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Bibliographic Details
Published in:International journal of radiation biology 2016-10, Vol.92 (10), p.558-562
Main Authors: Romero, Ivonne, Lamadrid, Ana Ilsa, González, Jorge Ernesto, Mandina, Tania, García, Omar
Format: Article
Language:English
Subjects:
Biological Assay - methods
Blood volume
Chromosome Aberrations - radiation effects
Culture Media - chemistry
culture time
cytogenetic
Cytogenetic Analysis - methods
Dose-Response Relationship, Radiation
Gamma Rays
Indicators and Reagents - chemistry
Lymphocytes - radiation effects
mass casualty scenario
PCC assay
Radiation Dosage
Radiation Exposure - analysis
Radiation Monitoring - methods
radiological accident
Reproducibility of Results
Sensitivity and Specificity
Space life sciences
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Summary:The possibility to reduce the culture time and volume of blood and reagents required for the chemical Premature Chromosome Condensation (PCC) assay is demonstrated in this work. Peripheral whole blood was exposed to gamma radiation (1-20 Gy). Lymphocytes were cultured for 40 h, using 50 μl of blood and 450 μl of culture medium. The dose-response curves were adjusted, using length ratio (LR) of the longest to the shortest chromosome piece, and the frequency of rings per cell. No statistical differences were found between the results obtained with this method and those reported with the classical PCC assay culture. The minimization of culture time and reagents in combination with the automatic measurement of the LR of the chromosome pieces, or the scoring of rings, can be a valuable biodosimetry tool in a mass casualty scenario.
ISSN:0955-3002
1362-3095
DOI:10.1080/09553002.2016.1206236