Expression Profiling of Innate Immune Genes in Milk Somatic Cells During Subclinical Mastitis in Crossbred Dairy Cows

Innate immune mechanism plays a key role in mammary defense, from recognition of pathogens to activation of nonspecific and specific immunity involved in elimination of pathogens. Expression profiles of innate immune response genes namely Toll like receptor 2 (TLR-2), Peptidoglycan recognition prote...

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Bibliographic Details
Published in:Animal biotechnology 2016-10, Vol.27 (4), p.303-309
Main Authors: Karthikeyan, A., Radhika, G., Aravindhakshan, T. V., Anilkumar, K.
Format: Article
Language:English
Subjects:
Animals
Candidate gene expression
Cattle
Cells, Cultured
crossbred cows
Cytokines - analysis
Cytokines - genetics
Cytokines - metabolism
Female
Gene Expression Profiling
Immunity, Innate - genetics
Immunity, Innate - immunology
Innate immune response
L-Selectin - analysis
L-Selectin - genetics
L-Selectin - metabolism
Mastitis, Bovine - immunology
Milk - cytology
Milk - immunology
Staphylococcus aureus
Subclinical mastitis
Toll-Like Receptor 2 - analysis
Toll-Like Receptor 2 - genetics
Toll-Like Receptor 2 - metabolism
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Summary:Innate immune mechanism plays a key role in mammary defense, from recognition of pathogens to activation of nonspecific and specific immunity involved in elimination of pathogens. Expression profiles of innate immune response genes namely Toll like receptor 2 (TLR-2), Peptidoglycan recognition protein 1 (PGLYRP-1), Interleukin 8 receptor (IL-8 R), L-Selectin (SELL), and Osteopontin (OPN) in milk somatic cells of subclinical mastitis (SCM) affected crossbred cows were investigated under this study at transcript level using quantitative real time polymerase chain reaction (qRT-PCR). Dairy cows in mid lactation were screened for SCM using California Mastitis Test (CMT), Somatic Cell Count (SCC) and Electrical Conductivity test (EC). Based on results of SCM screening tests, crossbred cows were clustered into two groups with four Staphylococcus aureus infected SCM cows and four apparently healthy cows. The expressions levels of TLR-2, PGLYRP-1, IL-8 R, SELL, and OPN in milk somatic cells of SCM affected cows were significantly higher (p 
ISSN:1049-5398
1532-2378
DOI:10.1080/10495398.2016.1184676