5-Lipoxygenase inhibitor zileuton inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages

Using Fura-2AM microfluorimetry, we have shown for the first time that 5-lipoxygenase specific inhibitor antiasthmatic agent zileuton significantly inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages. The results support 5-lipoxygenase involvement in the effect of glutoxim and m...

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Bibliographic Details
Published in:Doklady. Biochemistry and biophysics 2016-07, Vol.469 (1), p.302
Main Authors: Krutetskaya, Z I, Milenina, L S, Naumova, A A, Antonov, V G, Nozdrachev, A D
Format: Article
Language:English
Subjects:
Animals
Anti-Asthmatic Agents - pharmacology
Arachidonate 5-Lipoxygenase - metabolism
Calcium - metabolism
Calcium Signaling - drug effects
Calcium Signaling - physiology
Cations, Divalent - metabolism
Cells, Cultured
Cytophotometry
Drug Combinations
Drug Interactions
Fura-2 - analogs & derivatives
Hydroxyurea - analogs & derivatives
Hydroxyurea - pharmacology
Inosine - pharmacology
Intracellular Space - drug effects
Intracellular Space - enzymology
Lipoxygenase Inhibitors - pharmacology
Macrophages, Peritoneal - drug effects
Macrophages, Peritoneal - enzymology
Microscopy, Fluorescence
Oligopeptides - pharmacology
Rats, Wistar
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Summary:Using Fura-2AM microfluorimetry, we have shown for the first time that 5-lipoxygenase specific inhibitor antiasthmatic agent zileuton significantly inhibits Ca(2+)-responses induced by glutoxim and molixan in macrophages. The results support 5-lipoxygenase involvement in the effect of glutoxim and molixan on intracellular Ca(2+) concentration in macrophages and indicate the inadvisability of a combined use of drugs glutoxim and molixan and antiasthmatic agent zileuton.
ISSN:1608-3091
DOI:10.1134/S1607672916040177