Selection of specific inhibitor peptides in enzyme-linked immunosorbent assay (ELISA) of cardiac troponin I using immuno-dominant epitopes as competitor

Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed poly...

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Bibliographic Details
Published in:Journal of immunoassay & immunochemistry 2017, Vol.38 (1), p.72-81
Main Authors: Rezaee, Majid Asiabanha, Rasaee, Mohammad Javad, Mohammadnejad, Javad
Format: Article
Language:English
Subjects:
3D molecular structure
Binding, Competitive - immunology
Calcium-binding protein
cardiac troponin I
Dose-Response Relationship, Drug
Enzyme-linked immunosorbent assay
Enzyme-Linked Immunosorbent Assay - methods
Epitopes
Gold
Heart
Heart diseases
Humans
immunodominant epitopes
Immunodominant Epitopes - chemistry
Immunodominant Epitopes - immunology
Immunosorbents - chemistry
molecular dynamics
Molecular Dynamics Simulation
Myocardial infarction
Myocardial Infarction - diagnosis
Myocardial Infarction - immunology
Peptides
Peptides - chemistry
Peptides - immunology
polyclonal antibody
Sensitivity
Troponin
Troponin I
Troponin I - analysis
Troponin I - chemistry
Troponin I - immunology
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Summary:Human cardiac troponin I (cTni) is the gold marker for early diagnosis of myocardial infarction. In this regard, four immune-dominant epitopes of cTni were predicted and their 3D structures were determined. Thereafter, the competitive performance of the peptides was monitored with the developed polyclonal antibody-based indirect competitive ELISA; a half-maximal inhibitory concentration (IC50) of 0.49 (µg/mL) and detection limit of 0.037 (µg/mL) were achieved for recombinant cTni. The competitive ELISA determined sensitivity levels of 0.306, 0.141, 0.960, and 0.155 (µg/mL), respectively, for each peptide as competitor. We indicated that two of the selected epitopes have significant sensitivity scales and inhibition ability.
ISSN:1532-1819
1532-4230
DOI:10.1080/15321819.2016.1216444