Effect of Embryonic Tissue Immunization on Chemically Induced Gastrointestinal Tumors in Rats. I. Can Embryonic Antigens Act as Rejection Antigens?

Inbred WF rats were inoculated with crude suspensions prepared from liver and gut tissue of 12- to 15-day fetuses of the same strain. Rats previously unsensitized to syngeneic embryonic tissue were inoculated with fetal material sc three times during exposure to 1,2-dimethylhydrazine dihydrochloride...

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Bibliographic Details
Published in:JNCI : Journal of the National Cancer Institute 1978-07, Vol.61 (1), p.189-201
Main Authors: Bansal, Bharti R., Mark, Raymond, Rhoads, Jonathan E., Bansal, Subhash C.
Format: Article
Language:English
Subjects:
Adenocarcinoma - chemically induced
Adenocarcinoma - immunology
Animals
Antibodies, Neoplasm
Antigens - administration & dosage
Dimethylhydrazines
Fetus - immunology
Gastrointestinal Neoplasms - chemically induced
Gastrointestinal Neoplasms - immunology
Immunity
Methylhydrazines
Neoplasm Metastasis
Neoplasms, Experimental - immunology
Rats
Rats, Inbred WF
Skin - immunology
Time Factors
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Summary:Inbred WF rats were inoculated with crude suspensions prepared from liver and gut tissue of 12- to 15-day fetuses of the same strain. Rats previously unsensitized to syngeneic embryonic tissue were inoculated with fetal material sc three times during exposure to 1,2-dimethylhydrazine dihydrochloride (DMH), a gastrointestinal (Gl) carcinogen in rodents. Properly timed immunization inhibited the development, growth, and metastasis of primary Gl tumors. This effect was observed in both sexes; however, it was more pronounced in male rats. Nine WF rats with DMH-in-duced carcinoma of the Gl tract were inoculated sc with syngeneic fetal tissue. Of 9 rats, 7 rejected the embryonal tissue implants, which thus demonstrated the presence of a concomitant immune response to embryonic antigen(s). Two rats in which fetal tissue grew out to palpable nodules had multiple Gl tumors with metastasis and extra-Gl tumors, i.e., a massive tumor load. Ten other rats with DMH-induced Gl tumors were treated with unblocking serum. The unblocking serum was inoculated to counteract serum-blocking factors in vivo. These rats were inoculated intradermally with syngeneic fetal tissue. In all 10 rats, inflammation and necrosis were noted at the inoculation site after 24–72 hours, which thus demonstrated a delayed hypersensitivity reaction to embryonic antigens. Our experiments suggest that embryonic antigens common to fetal and tumor cells can induce immunity in an autochthonous host and can act as rejection antigens.
ISSN:0027-8874
1460-2105
DOI:10.1093/jnci/61.1.189