Phytochemical screening and analgesic profile of the lyophilized aqueous extract obtained from Chrysobalanus icaco leaves in experimental protocols

• Published in: Pharmaceutical biology 2016-12, Vol.54 (12), p.3055-3062
• Main Authors: Araújo-Filho, Heitor G., Dias, Jessica Deise Santos, Quintans-Júnior, Lucindo J., Santos, Márcio R. V., White, Pollyanna A. S., Barreto, Rosana S. S., Barreto, André S., Estevam, Charles S., Araujo, Silvan S., Almeida, Jackson R. G. S., Menezes, Irwin R. A., Coutinho, Henrique D. M., Quintans, Jullyana S. S.
• Format: Article
• Language: English
• Subjects: Analgesics - isolation & purification; Analgesics - pharmacology; Animals; Anti-hyperalgesic; anti-nflammatory; anti-nociceptive; Chrysobalanaceae; Dose-Response Relationship, Drug; Drug Evaluation, Preclinical - methods; Freeze Drying; Male; Mice; Pain Measurement - drug effects; Pain Measurement - methods; Phytochemicals - isolation & purification; Phytochemicals - pharmacology; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Plant Leaves; terpenes; TNF-α; Water - pharmacology
• ISSN: 1388-0209; 1744-5116
• DOI: 10.1080/13880209.2016.1204618

Context: Chrysobalanus icaco L. (Chrysobalanaceae) has been used for the treatment of abdominal pain and cramps. Objective: Assess the chemical and pharmacological profile of the lyophilized aqueous extract from C. icaco leaves (AEC). Materials and methods: Chromatographic methods were used to assess compounds from AEC. Mice were treated with vehicle (control group) or AEC (100, 200 or 400 mg/kg, p.o.) (group with 7-8 mice) and the analgesic profile was assessed employing the acetic acid-induced writhing, formalin, hot plate tests and hyperalgesia induced by carrageenan (CG) or tumour necrosis factor-alpha. The animal motor performance was assessed using rota-rod and grip strength tests. Results: The chromatographic profile of AEC demonstrated the presence of terpenoid compounds. The acute pretreatment with AEC, at all doses, produced a significant (p