Molecular mechanisms underlying histological and biochemical changes induced by nitrate in rat liver and the efficacy of S-Allylcysteine

This study was designed to investigate structural and ultra-structural changes induced by nitrate in rat liver, examine their molecular basis, and evaluate the possible protective role of S-Allylcysteine (SAC). Adult male albino rats were assigned to: control, SAC, nitrate, and nitrate+ SAC groups....

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Bibliographic Details
Published in:Ultrastructural pathology 2017-01, Vol.41 (1), p.10-22
Main Authors: Kattaia, Asmaa A. A., Abd El-Baset, Samia A., Mohamed, Eman M., Abdul-Maksou, Rehab S., Elfakharany, Yara M.
Format: Article
Language:English
Subjects:
Alanine Transaminase - blood
Animals
Anti-Inflammatory Agents - pharmacology
Antioxidants - pharmacology
Apoptosis - drug effects
Aspartate Aminotransferases - blood
Biomarkers - blood
Chemical and Drug Induced Liver Injury - blood
Chemical and Drug Induced Liver Injury - genetics
Chemical and Drug Induced Liver Injury - pathology
Chemical and Drug Induced Liver Injury - prevention & control
Cysteine - analogs & derivatives
Cysteine - pharmacology
Cytoprotection
Histopathology
HSP70 Heat-Shock Proteins - genetics
HSP70 Heat-Shock Proteins - metabolism
Liver - drug effects
Liver - metabolism
Liver - ultrastructure
Liver Cirrhosis, Experimental - blood
Liver Cirrhosis, Experimental - genetics
Liver Cirrhosis, Experimental - pathology
Liver Cirrhosis, Experimental - prevention & control
Male
Microscopy, Electron, Transmission
molecular mechanisms
nitrate
Nitrates
Oxidative Stress - drug effects
Proto-Oncogene Proteins c-bcl-2 - genetics
Proto-Oncogene Proteins c-bcl-2 - metabolism
rat liver
Rats
Real-Time Polymerase Chain Reaction
Reverse Transcriptase Polymerase Chain Reaction
S-Allylcysteine
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Summary:This study was designed to investigate structural and ultra-structural changes induced by nitrate in rat liver, examine their molecular basis, and evaluate the possible protective role of S-Allylcysteine (SAC). Adult male albino rats were assigned to: control, SAC, nitrate, and nitrate+ SAC groups. Serum ALT and AST were measured. Liver samples were processed for light and electron microscope examinations, biochemical analysis, real-time PCR, and immunohistochemistry of heat shock protein 70 (HSP70) and Bcl-2. Nitrate induced histopathological and biochemical alterations in rat liver. The underlying mechanisms included nitrosative and oxidative stress, inflammation, fibrosis, and apoptosis that are alleviated by SAC.
ISSN:0191-3123
1521-0758
DOI:10.1080/01913123.2016.1252821