Arabinoxylan hydrolyzates as immunomodulators in Caco-2 and HT-29 colon cancer cell lines

• Published in: Food & function 2017-01, Vol.8 (1), p.22-231
• Main Authors: Mendis, Mihiri, Leclerc, Estelle, Simsek, Senay
• Format: Article
• Language: English
• Subjects: Caco-2 Cells; Colonic Neoplasms - drug therapy; Colonic Neoplasms - genetics; Colonic Neoplasms - immunology; Endo-1,4-beta Xylanases - chemistry; Glycoside Hydrolases - chemistry; HT29 Cells; Humans; Hydrolysis; Immunologic Factors - chemistry; Immunologic Factors - pharmacology; Interleukin-8 - genetics; Interleukin-8 - immunology; Triticum aestivum; Tumor Necrosis Factor-alpha - genetics; Tumor Necrosis Factor-alpha - immunology; Xylans - chemistry; Xylans - pharmacology
• ISSN: 2042-6496; 2042-650X
• DOI: 10.1039/c6fo00866f

The use of plant derived polysaccharides as health promoters has gained immense interest in the past few years. Arabinoxylan (AX) is the predominant non-starch polysaccharide in cereals and grasses including wheat. The current research aimed to investigate the structure-function relationship of arabinoxylan hydrolyzates (AXH), obtained by the enzymatic hydrolysis of AX using xylanase and arabinofuranosidase as immunomodulators in two colon cancer cell lines: Caco-2 and HT-29. Fine structural details had a strong correlation with the immunological properties of the wheat AXH. As a general trend, as the presence of arabinose substitution increased in the AXH, the production of proinflammatory cytokines, IL-8 and TNF-α, decreased in both cell lines. Thus, AXH with a higher degree of arabinose substitution might be better adept in lowering inflammation in colon cancer cells. Structure-function relationship of wheat derived arabinoxylan hydrolyzates as immunomodulators was investigated using intestinal cell lines. Fine structural details had a strong correlation with the immunological properties of the wheat arabinoxylan hydrolyzates.