The Detection of Soluble ABH Blood Group Substances in Semen and Saliva Using Monoclonal Blood Grouping Reagents

Using an enzyme-linked immunosorbent assay (ELISA), this study investigated the use of monoclonal antibodies for detecting secreted ABH blood group substances in semen and saliva. The results demonstrated that the behavior of some monoclonals were unpredictable and often failed to detect the corresp...

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Bibliographic Details
Published in:Journal of forensic sciences 1989-09, Vol.34 (5), p.1082-1089
Main Author: Mudd, JL
Format: Article
Language:English
Subjects:
ABO Blood-Group System
Antibodies, Monoclonal
Body fluids
Enzyme-Linked Immunosorbent Assay
Forensic hematology
Humans
Indicators and Reagents
Male
Phenotype
Saliva - analysis
Semen - analysis
Solubility
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Summary:Using an enzyme-linked immunosorbent assay (ELISA), this study investigated the use of monoclonal antibodies for detecting secreted ABH blood group substances in semen and saliva. The results demonstrated that the behavior of some monoclonals were unpredictable and often failed to detect the corresponding antigen in a number of the specimens tested. The suitability of the monoclonal reagents for detecting soluble blood group antigens could not be predicted by their behavior with red cell antigens. Consequently, care must be taken in the selection of monoclonal reagents for use in the detection of secreted blood group antigens.
ISSN:0022-1198
1556-4029
DOI:10.1520/JFS12744J