Effects of astaxanthin on oxidative stress induced by Cu 2+ in prostate cells

Astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dim...

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Published in:Journal of Zhejiang University. B. Science 2017-02, Vol.18 (2), p.161
Main Authors: Meng, Hong-Zhou, Ni, Xiao-Feng, Yu, Hai-Ning, Wang, Shan-Shan, Shen, Sheng-Rong
Format: Article
Language:English
Subjects:
Antioxidants - chemistry
Catalase - metabolism
Cell Line, Tumor
Cell Survival
Copper - chemistry
Fibrinolytic Agents - chemistry
Flow Cytometry
Glutathione Peroxidase - metabolism
Humans
Ions
Male
Malondialdehyde - metabolism
Membrane Potential, Mitochondrial
Mitochondria - metabolism
Oxidative Stress
Prostate - drug effects
Prostatic Neoplasms - drug therapy
Reactive Oxygen Species - metabolism
Superoxide Dismutase - metabolism
Xanthophylls - chemistry
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Summary:Astaxanthin (AST), a carotenoid molecule extensively found in marine organisms and increasingly used as a dietary supplement, has been reported to have beneficial effects against oxidative stress. In the current paper, the effects of AST on viability of prostate cells were investigated by 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyltetrazolium bromide (MTT) assay; cell apoptosis and intracellular reactive oxygen species (ROS) levels were determined by flow cytometry; the mitochondrial membrane potential (MMP) was measured by fluorospectrophotometer; and activities of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GSH-Px) were evaluated by a detection kit. The results show that copper ion (Cu ) induced apoptosis, along with the accumulation of intracellular ROS and MDA, in both prostate cell lines (RWPE-1 and PC-3). AST treatments could decrease the MDA levels, increase MMP, and keep ROS stable in RWPE-1 cell line. An addition of AST decreased the SOD, GSH-Px, and CAT activities in PC-3 cell line treated with Cu , but had a contrary reaction in RWPE-1 cell lines. In conclusion, AST could contribute to protecting RWPE-1 cells against Cu -induced injuries but could cause damage to the antioxidant enzyme system in PC-3 cells.
ISSN:1862-1783
DOI:10.1631/jzus.B1500296