Optical interference probe of biofilm hydrology: label-free characterization of the dynamic hydration behavior of native biofilms

Biofilm produced by Escherichia coli (E. coli) or Pseudomonas aeruginosa (P. aeruginosa) on quartz or polystyrene is removed from the culture medium and drained. Observed optical interference fringes indicate the presence of a layer of uniform thickness with refractive index different from air-dried...

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Bibliographic Details
Published in:Journal of biomedical optics 2017-03, Vol.22 (3), p.035003-035003
Main Authors: McDonough, Richard T, Zheng, Hewen, Alila, Mercy A, Goodisman, Jerry, Chaiken, Joseph
Format: Article
Language:English
Subjects:
Biofilms
Escherichia coli - physiology
Microbiological Techniques - methods
Microscopy, Confocal
Optical Imaging
Pseudomonas aeruginosa - physiology
Water - metabolism
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Summary:Biofilm produced by Escherichia coli (E. coli) or Pseudomonas aeruginosa (P. aeruginosa) on quartz or polystyrene is removed from the culture medium and drained. Observed optical interference fringes indicate the presence of a layer of uniform thickness with refractive index different from air-dried biofilm. Fringe wavelengths indicate that layer optical thickness is
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.22.3.035003