Detection of blood volumes and haemoglobin mass by means of CO re-breathing and indocyanine green and sodium fluorescein injections

The main aim of the present study was to quantify the magnitude of differences introduced when estimating a given blood volume compartment (e.g. plasma volume) through the direct determination of another compartment (e.g. red cell volume) by multiplication of venous haematocrit and/or haemoglobin co...

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Bibliographic Details
Published in:Scandinavian journal of clinical and laboratory investigation 2017-04, Vol.77 (3), p.164-174
Main Authors: Keiser, Stefanie, Meinild-Lundby, Anne-Kristine, Steiner, Thomas, Trösch, Severin, Rauber, Sven, Krafft, Alexander, Burkhardt, Tilo, Hilty, Matthias Peter, Siebenmann, Christoph, Wehrlin, Jon Peter, Lundby, Carsten
Format: Article
Language:English
Subjects:
Administration, Inhalation
Adult
Analysis of Variance
Blood Volume
Carbon monoxide
Carbon Monoxide - metabolism
Cell Size
Erythrocytes - cytology
Erythrocytes - physiology
Fluorescein - pharmacokinetics
haematocrit
HbCO
Hematocrit
Hemoglobins - analysis
Humans
Indocyanine Green - pharmacokinetics
Male
plasma volume
red blood cell volume
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Summary:The main aim of the present study was to quantify the magnitude of differences introduced when estimating a given blood volume compartment (e.g. plasma volume) through the direct determination of another compartment (e.g. red cell volume) by multiplication of venous haematocrit and/or haemoglobin concentration. However, since whole body haematocrit is higher than venous haematocrit such an approach might comprise certain errors. To test this experimentally, four different methods for detecting blood volumes and haemoglobin mass (Hb mass ) were compared, namely the carbon monoxide (CO) re-breathing (for Hb mass ), the indocyanine green (ICG; for plasma volume [PV]) and the sodium fluorescein (SoF; for red blood cell volume [RBCV]) methods. No difference between ICG and CO re-breathing derived PV could be established when a whole body/venous haematocrit correction factor of 0.91 was applied (p = 0.11, r = 0.43, mean difference −340 ± 612 mL). In contrast, when comparing RBCV derived by the CO re-breathing and the SoF method, the SoF method revealed lower RBCV values as compared to the CO re-breathing method (p 
ISSN:0036-5513
1502-7686
1502-7686
DOI:10.1080/00365513.2016.1271908