An mRNA Capping Enzyme Targets FACT to the Active Gene To Enhance the Engagement of RNA Polymerase II into Transcriptional Elongation

• Published in: Molecular and cellular biology 2017-07, Vol.37 (13)
• Main Authors: Sen, Rwik, Kaja, Amala, Ferdoush, Jannatul, Lahudkar, Shweta, Barman, Priyanka, Bhaumik, Sukesh R.
• Format: Article
• Language: English
• Subjects: Acid Anhydride Hydrolases - genetics; Acid Anhydride Hydrolases - metabolism; Alcohol Dehydrogenase - genetics; Alcohol Dehydrogenase - metabolism; Binding Sites; Cet1; Chromatin - genetics; DNA-Binding Proteins - genetics; DNA-Binding Proteins - metabolism; FACT; High Mobility Group Proteins - genetics; High Mobility Group Proteins - metabolism; mRNA capping; Promoter Regions, Genetic; RNA polymerase II; RNA Polymerase II - genetics; RNA Polymerase II - metabolism; RNA, Messenger; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - growth & development; Saccharomyces cerevisiae - metabolism; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; transcription; Transcription Elongation, Genetic; Transcriptional Elongation Factors - genetics; Transcriptional Elongation Factors - metabolism
• ISSN: 1098-5549; 0270-7306; 1098-5549
• DOI: 10.1128/MCB.00029-17

We have recently demonstrated that an mRNA capping enzyme, Cet1, impairs promoter-proximal accumulation/pausing of RNA polymerase II (Pol II) independently of its capping activity in Saccharomyces cerevisiae to control transcription. However, it is still unknown how Pol II pausing is regulated by Cet1. Here, we show that Cet1's N-terminal domain (NTD) promotes the recruitment of FACT ( fa cilitates c hromatin t ranscription that enhances the engagement of Pol II into transcriptional elongation) to the coding sequence of an active gene, ADH1, independently of mRNA-capping activity. Absence of Cet1's NTD decreases FACT targeting to ADH1 and consequently reduces the engagement of Pol II in transcriptional elongation, leading to promoter-proximal accumulation of Pol II. Similar results were also observed at other genes. Consistently, Cet1 interacts with FACT. Collectively, our results support the notion that Cet1's NTD promotes FACT targeting to the active gene independently of mRNA-capping activity in facilitating Pol II's engagement in transcriptional elongation, thus deciphering a novel regulatory pathway of gene expression.