Studies on lipid A isolated from Phyllobacterium trifolii PETP02 T lipopolysaccharide

The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02 , a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-D-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, subst...

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Bibliographic Details
Published in:Antonie van Leeuwenhoek 2017-11, Vol.110 (11), p.1413
Main Authors: Zamlynska, Katarzyna, Komaniecka, Iwona, Zebracki, Kamil, Mazur, Andrzej, Sroka-Bartnicka, Anna, Choma, Adam
Format: Article
Language:English
Subjects:
Fatty Acids - analysis
Glucosamine - analogs & derivatives
Glucosamine - chemistry
Hexuronic Acids - chemistry
Lipid A - biosynthesis
Lipid A - chemistry
Lipid A - isolation & purification
Lipopolysaccharides - chemistry
Lipopolysaccharides - isolation & purification
Magnetic Resonance Spectroscopy
Mesorhizobium - chemistry
Mesorhizobium - genetics
Metabolic Networks and Pathways - genetics
Phyllobacteriaceae - chemistry
Phyllobacteriaceae - genetics
Sequence Homology, Amino Acid
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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Summary:The structure of lipid A from lipopolysaccharide of Phyllobacterium trifolii PETP02 , a nitrogen-fixing symbiotic bacterium, was studied. It was found that the lipid A backbone was composed of two 2,3-diamino-2,3-dideoxy-D-glucose (GlcpN3N) residues connected by a β-(1 → 6) glycosidic linkage, substituted by galacturonic acid (GalpA) at position C-1 and partly decorated by a phosphate residue at C-4' of the non-reducing GlcpN3N. Both diaminosugars were symmetrically substituted by 3-hydroxy fatty acids (14:0(3-OH) and 16:0(3-OH)). Ester-linked secondary acyl residues [i.e. 19:0cyc and 28:0(27-OH) or 28:0(27-4:0(3-OMe))] were located in the distal part of lipid A. A high similarity between the lipid A of P. trifolii and Mesorhizobium was observed and discussed from the perspective of the genetic context of both genomes.
ISSN:1572-9699