Isolation of the Gene and Hypothalamic cDNA for the Common Precursor of Gonadotropin-Releasing Hormone and Prolactin Release-Inhibiting Factor in Human and Rat

Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolacting release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species...

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Published in:Proceedings of the National Academy of Sciences - PNAS 1986-01, Vol.83 (1), p.179-183
Main Authors: Adelman, John P., Mason, Anthony J., Hayflick, Joel S., Seeburg, Peter H.
Format: Article
Language:English
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Summary:Cloned cDNAs encoding the precursor protein for gonadotropin-releasing hormone (Gn-RH) and prolacting release-inhibiting factor (PIF) were isolated from libraries derived from human and rat hypothalamic mRNA. Nucleotide sequence analyses predict precursor proteins of 92 amino acids for both species and show identity between the human placental and human hypothalamic precursor proteins. Whereas the Gn-RH peptide structure is completely conserved in human and rat, the PIF domain of the precursor displays 70% interspecies homology. Genomic analyses revealed the presence of a single Gn-RH-PIF gene in human and rat containing sequences corresponding to the cDNA distributed across four exons.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.83.1.179