Increased Aβ 42 -α7-like nicotinic acetylcholine receptor complex level in lymphocytes is associated with apolipoprotein E4-driven Alzheimer's disease pathogenesis

• Published in: Alzheimer's research & therapy 2017-07, Vol.9 (1), p.54
• Main Authors: Wang, Hoau-Yan, Trocmé-Thibierge, Caryn, Stucky, Andres, Shah, Sanket M, Kvasic, Jessica, Khan, Amber, Morain, Philippe, Guignot, Isabelle, Bouguen, Eva, Deschet, Karine, Pueyo, Maria, Mocaer, Elisabeth, Ousset, Pierre-Jean, Vellas, Bruno, Kiyasova, Vera
• Format: Article
• Language: English
• Subjects: Aged; Aged, 80 and over; alpha7 Nicotinic Acetylcholine Receptor - metabolism; Alzheimer Disease - genetics; Alzheimer Disease - pathology; Amyloid beta-Peptides - metabolism; Amyloid beta-Peptides - pharmacology; Animals; Cognitive Dysfunction - genetics; Cognitive Dysfunction - pathology; Dose-Response Relationship, Drug; Female; Frontal Lobe - ultrastructure; Humans; Lymphocytes - drug effects; Lymphocytes - metabolism; Male; Peptide Fragments - metabolism; Peptide Fragments - pharmacology; Phosphorylation - drug effects; Protein Binding - drug effects; Rats; Rats, Sprague-Dawley; Receptors, LDL - metabolism; Statistics as Topic; Synaptosomes - metabolism; Synaptosomes - ultrastructure; tau Proteins - metabolism
• ISSN: 1758-9193

The apolipoprotein E ε4 (APOE4) genotype is a prominent late-onset Alzheimer's disease (AD) risk factor. ApoE4 disrupts memory function in rodents and may contribute to both plaque and tangle formation. Coimmunoprecipitation and Western blot detection were used to determine: 1) the effects of select fragments from the apoE low-density lipoprotein (LDL) binding domain and recombinant apoE subtypes on amyloid beta (Aβ) -α7 nicotinic acetylcholine receptor (α7nAChR) interaction and tau phosphorylation in rodent brain synaptosomes; and 2) the level of Aβ -α7nAChR complexes in matched controls and patients with mild cognitive impairment (MCI) and dementia due to AD with known APOE genotypes. In an ex vivo study using rodent synaptosomes, apoE of the apoE promotes Aβ -α7nAChR association and Aβ -induced α7nAChR-dependent tau phosphorylation. In a single-blind study, we examined lymphocytes isolated from control subjects, patients with MCI and dementia due to AD with known APOE genotypes, sampled at two time points (1 year apart). APOE ε4 genotype was closely correlated with heightened Aβ -α7nAChR complex levels and with blunted exogenous Aβ effects in lymphocytes derived from AD and MCI due to AD cases. Similarly, plasma from APOE ε4 carriers enhanced the Aβ -induced Aβ -α7nAChR association in rat cortical synaptosomes. The progression of cognitive decline in APOE ε4 carriers correlated with higher levels of Aβ -α7nAChR complexes in lymphocytes and greater enhancement by their plasma of Aβ -induced Aβ -α7nAChR association in rat cortical synaptosomes. Our data suggest that increased lymphocyte Aβ -α7nAChR-like complexes may indicate the presence of AD pathology especially in APOE ε4 carriers. We show that apoE, especially apoE4, promotes Aβ -α7nAChR interaction and Aβ -induced α7nAChR-dependent tau phosphorylation via its apoE domain These apoE-mediated effects may contribute to the APOE ε4-driven neurodysfunction and AD pathologies.