Post-treatment of melatonin with CCl 4 better reduces fibrogenic and oxidative changes in liver than melatonin co-treatment

Therapeutic effects of melatonin (MEL) in targeting CCl -induced liver fibrosis has been widely known, but there is no study comparing oxidative and fibrogenic changes in co- and post-treatment of MEL with CCl , which was further aimed in this experiment. Male SD rats were injected with CCl (1 mL/kg...

Full description

Saved in:
Bibliographic Details
Published in:Journal of cellular biochemistry 2018-02, Vol.119 (2), p.1716
Main Authors: Mortezaee, Keywan, Majidpoor, Jamal, Daneshi, Erfan, Abouzaripour, Morteza, Abdi, Mahdad
Format: Article
Language:English
Subjects:
Alanine Transaminase - blood
Animals
Carbon Tetrachloride - adverse effects
Disease Models, Animal
Gene Expression Regulation - drug effects
Hydroxyproline - metabolism
Injections, Intraperitoneal
Lipid Metabolism - drug effects
Liver Cirrhosis - chemically induced
Liver Cirrhosis - drug therapy
Liver Cirrhosis - metabolism
Male
Malondialdehyde - blood
Melatonin - administration & dosage
Melatonin - pharmacology
Oxidative Stress - drug effects
Rats
Rats, Sprague-Dawley
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Therapeutic effects of melatonin (MEL) in targeting CCl -induced liver fibrosis has been widely known, but there is no study comparing oxidative and fibrogenic changes in co- and post-treatment of MEL with CCl , which was further aimed in this experiment. Male SD rats were injected with CCl (1 mL/kg/i.p./daily) dissolved 1:1 in olive oil for 1 month. Some animals received MEL (20 mg/kg/i.p./daily) diluted in 1 mL PBS in combination with CCl (co-treatment), and some rats were treated with MEL, beginning with injection of the last dose of CCl for one month (post-treatment). The groups were control, CCl , CCl -co vehicle, CCl -post vehicle, post-CCl , MEL co-treatment, and MEL post-treatment. MEL post-treatment group showed significantly lower lipid deposition, serum malondialdehyde (MDA), serum alanine aminotransferase (ALT), and liver hydroxyproline. This group also had low expressions of Bax and transforming growth factor-β1 (TGF-β1). MEL post-treatment group revealed higher sera levels of albumin, superoxide dismutase (SOD) and glutathione peroxidase (GPx). Expression levels of metalloproteinase-13 (MMP-13) and Bcl2 was also higher in this group (P ≤ 0.05 vs co-treatment). Results of the present study indicated that MEL post-treatment is more powerful in reduction of CCl -induced liver fibrosis through reduction of oxidative stress and maintenance of matrix balance.
ISSN:1097-4644