Isolation of a novel compound (MIMO2) from the methanolic extract of Moringa oleifera leaves: protective effects against vanadium-induced cytotoxity

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2018-07, Vol.41 (3), p.249-258
• Main Authors: Igado, Olumayowa O., Glaser, Jan, Ramos-Tirado, Mario, Bankoğlu, Ezgi Eylül, Atiba, Foluso A., Holzgrabe, Ulrike, Stopper, Helga, Olopade, James O.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - isolation & purification; Antioxidants - pharmacology; Cells, Cultured; Chromatography, High Pressure Liquid; Comet assay; Cytoprotection; dihydroethidium; DNA Damage; ferric-reducing antioxidant potential; Mice; MIMO2; Moringa oleifera; Moringa oleifera - chemistry; Plant Extracts - analysis; Plant Extracts - pharmacology; Plant Leaves - chemistry; Reactive Oxygen Species - metabolism; vanadium; Vanadium - toxicity
• ISSN: 0148-0545; 1525-6014
• DOI: 10.1080/01480545.2017.1366504

Moringa oleifera is reported to be a miracle plant, with positive effects on practically every system in the animal body. The methanolic extract of Moringa oleifera leaves was fractionated using liquid-liquid fractionation, column chromatography and preparative high-performance liquid chromatography (HPLC). Bioassay guided fractionation using Ferric Reducing Antioxidant Power (FRAP) was used to determine the fraction with the highest antioxidative power. Chemical structure was elucidated with nuclear magnetic resonance (NMR) spectroscopy. FRAP showed that the pure compound, butyl p-hydroxyphenyl-acetate (MIMO2) exhibited an antioxidant activity higher than TEMPOL (positive control). Vanadium is a metal, which as a salt has been shown to be a neurotoxicant; and was therefore used to assess the efficacy of MIMO2 in this experiment. HT22 (immortalized mouse hippocampal) cells were used for cell culture. The Comet assay showed a statistically significant reduction (p