The expression of mRNA for peptidylarginine deiminase type 2 and type 4 in bone marrow CD34+ cells in rheumatoid arthritis

Antibodies directed to citrullinated proteins are highly specific for rheumatoid arthritis (RA). Citrullination is catalyzed by peptidylarginine deiminase (PAD) enzymes. The current study examined the mRNA expression of PADI2 and PADI4 in bone marrow (BM) CD34+ cells from RA patients. CD34+ cells we...

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Bibliographic Details
Published in:Clinical and experimental rheumatology 2018-03, Vol.36 (2), p.248
Main Authors: Nagai, Tatsuo, Matsueda, Yu, Tomita, Tetsuya, Yoshikawa, Hideki, Hirohata, Shunsei
Format: Article
Language:English
Subjects:
Adult
Aged
Aged, 80 and over
Antigens, CD34 - analysis
Arthritis, Rheumatoid - drug therapy
Arthritis, Rheumatoid - enzymology
Bone Marrow Cells - enzymology
Female
Humans
Male
Methotrexate - therapeutic use
Middle Aged
Protein-Arginine Deiminase Type 2
Protein-Arginine Deiminase Type 4
Protein-Arginine Deiminases - genetics
RNA, Messenger - analysis
Sp1 Transcription Factor - genetics
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Summary:Antibodies directed to citrullinated proteins are highly specific for rheumatoid arthritis (RA). Citrullination is catalyzed by peptidylarginine deiminase (PAD) enzymes. The current study examined the mRNA expression of PADI2 and PADI4 in bone marrow (BM) CD34+ cells from RA patients. CD34+ cells were purified from BM samples obtained from 48 RA patients and from 30 osteoarthritis (OA) patients during joint operations via aspiration from the iliac crest. The expression of mRNAs for PADI2, PADI4 and Sp1 was examined by quantitative reverse transcription PCR. The expression of mRNA for PADI2 was significantly higher in RA BM CD34+ cells than OA BM CD34+ cells. The expression of mRNAs for PADI4 and Sp1 in RA BM CD34+ cells appeared to be increased compared to OA BM CD34+ cells, although it did not reach the statistical significance. The levels of mRNAs for PADI2, PADI4 and Sp1 were not correlated with serum C-reactive protein or with the administration of methotrexate or oral steroids. Finally, the level of PADI2 mRNA as well as that of PADI4 mRNA was significantly correlated with the level of Sp1 mRNA in RA BM CD34+ cells. These results indicate that the mRNA expression of PADI2, PADI4 and Sp1 is upregulated in RA BM CD34+ cells independently of the systemic inflammation or treatment regimen. Moreover, the data suggest that the enhanced mRNA expression of PADI2 and PADI4 in BM CD34+ cells might be a result of the enhanced expression of Sp1 gene in RA BM CD34+ cells.
ISSN:0392-856X