Species Distribution of a Phosphoprotein (Parafusin) Involved in Exocytosis

A cytosolic phosphoprotein that appears to function in membrane fusion during exocytosis of secretory products has previously been isolated from Paramecium tetraurelia. This phosphoprotein, parafusin, with Mr 63,000, is rapidly dephosphorylated via a Ca2+-dependent process when secretagogues induce...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1989-02, Vol.86 (3), p.930-932
Main Authors: Satir, B. H., Hamasaki, T., Reichman, M., Murtaugh, T. J.
Format: Article
Language:English
Subjects:
Animals
Antibodies
Biological and medical sciences
Cell lines
Cell membranes
Cell physiology
Cells
Cross Reactions
dephosphorylation
Eukaryotic cells
Exocytosis
Fundamental and applied biological sciences. Psychology
Humans
Immunoblotting
Immunology
membrane fusion
Molecular and cellular biology
Molecular Weight
Molecules
parafusin
Paramecium - physiology
Phosphoproteins
Phosphoproteins - isolation & purification
Phosphoproteins - physiology
proteins
Rats
Secretion. Exocytosis
Species Specificity
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Summary:A cytosolic phosphoprotein that appears to function in membrane fusion during exocytosis of secretory products has previously been isolated from Paramecium tetraurelia. This phosphoprotein, parafusin, with Mr 63,000, is rapidly dephosphorylated via a Ca2+-dependent process when secretagogues induce exocytosis in competent cells. Dephosphorylation does not occur in exocytosis-incompetent cells. Polyclonal antibodies against purified parafusin have now been used to show that this protein is present in unicellular organisms and cells of metazoan groups of wide evolutionary divergence, such as yeast, insects, and mammals, including humans. These results suggest that parafusin was present early in the history of eukaryotes and that it is of functional importance in the general mechanism of exocytosis and membrane fusion.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.86.3.930