Structure of Genes for Human Growth Hormone and Chorionic Somatomammotropin

A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human...

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Bibliographic Details
Published in:Proceedings of the National Academy of Sciences - PNAS 1979-09, Vol.76 (9), p.4294-4298
Main Authors: Fiddes, John C., Seeburg, Peter H., DeNoto, Frances M., Hallewell, Robert A., Baxter, John D., Goodman, Howard M.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Bacteriophages
Base Sequence
Complementary DNA
DNA
DNA, Recombinant
Enzymes
Gels
Genes
Genetic vectors
Growth Hormone - genetics
Hormones
Humans
Introns
Nucleic Acid Precursors - genetics
Packaging
Placental Lactogen - genetics
RNA, Messenger - genetics
Transcription, Genetic
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Summary:A 2.6-kilobase (kb) EcoRI restriction endonuclease fragment containing human growth hormone (hGH; somatotropin) gene sequences and a 2.8-kb EcoRI fragment containing human chorionic somatomammotropin (hCS; choriomammotropin) gene sequences have been identified by hybridization to cloned cDNA. Human DNA was cleaved with EcoRI and fractionated by preparative agarose gel electrophoresis; DNA in the size range 2-3 kb was ligated to λ gt WES· λ B DNA and viable recombinant bacteriophage were recovered by in vitro packaging. After infection of Escherichia coli and screening of phage plaques, single isolates of hGH and hCS gene sequences were obtained. Restriction endonuclease mapping showed that the hGH gene contains three intervening sequences interrupting the coding sequence. Partial DNA sequence analysis of the hGH gene, obtained by the chain termination method, confirmed the location of the intervening sequences and the identity of the fragment.
ISSN:0027-8424
1091-6490
DOI:10.1073/pnas.76.9.4294