Vanadium pentoxide increased PTEN and decreased SHP1 expression in NK-92MI cells, affecting PI3K-AKT-mTOR and Ras-MAPK pathways

Vanadium is an air pollutant that imparts immunosuppressive effects on NK cell immune responses, in part, by dysregulating interleukin (IL)-2/IL-2R-mediated JAK signaling pathways and inducing apoptosis. The aim of the present study was to evaluate effects of vanadium pentoxide (V 2 O 5 ) on other I...

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Published in:Journal of immunotoxicology 2018-01, Vol.15 (1), p.1-11
Main Authors: Gallardo-Vera, Francisco, Tapia-Rodriguez, Miguel, Diaz, Daniel, Fortoul van der Goes, Teresa, Montaño, Luis F., Rendón-Huerta, Erika P.
Format: Article
Language:English
Subjects:
Air Pollution - adverse effects
Apoptosis
Cell Line
Extracellular Signal-Regulated MAP Kinases - metabolism
Gene Expression Regulation
Humans
IL-2
IL-2R
Killer Cells, Natural - physiology
NK cells
Oncogene Protein v-akt - metabolism
Phosphatidylinositol 3-Kinases - metabolism
Protein Tyrosine Phosphatase, Non-Receptor Type 6 - genetics
Protein Tyrosine Phosphatase, Non-Receptor Type 6 - metabolism
PTEN
PTEN Phosphohydrolase - genetics
PTEN Phosphohydrolase - metabolism
ras Proteins - metabolism
SHP1
Signal Transduction
signaling pathway
TOR Serine-Threonine Kinases - metabolism
Vanadium Compounds - toxicity
Vanadium pentoxide
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Summary:Vanadium is an air pollutant that imparts immunosuppressive effects on NK cell immune responses, in part, by dysregulating interleukin (IL)-2/IL-2R-mediated JAK signaling pathways and inducing apoptosis. The aim of the present study was to evaluate effects of vanadium pentoxide (V 2 O 5 ) on other IL-2 receptor-mediated signaling pathways, i.e. PI3K-AKT-mTOR and Ras-MAPK. Here, IL-2-independent NK-92MI cells were exposed to different V 2 O 5 doses for 24 h periods. Expression of PI3K, Akt, mTOR, ERK1/2, MEK1, PTEN, SHP1, BAD and phosphorylated forms, as well as caspases-3, -8, -9, BAX and BAK in/on the cells were then determined by flow cytometry. The results show that V 2 O 5 was cytotoxic to NK cells in a dose-related manner. Exposure increased BAD and pBAD expression and decreased that of BAK and BAX, but cell death was not related to caspase activation. At 400 µM V 2 O 5 , expression of PI3K-p85 regulatory subunit increased 20% and pPI3K 50%, while that of the non-pPI3K 110α catalytic subunit decreased by 20%. At 200 μM, V 2 O 5 showed significant decrease in non-pAkt expression (p 
ISSN:1547-691X
1547-6901
DOI:10.1080/1547691X.2017.1404662