L-Cysteine Conjugated Ruthenium Hydrous Oxide Nanomaterials with Anti-cancer Active Application

• Published in: Langmuir 2017-12
• Main Authors: Ganguly, Bichitra Nandi, Maity, Buddhadeb, Maity, Tapan Kumar, Manna, Joydeb, Roy, Madhusudan, Mukherjee, Manabendra, Debnath, Sushanta, Saha, Partha, Shilpa, Nagaraju, Rana, Rohit Kumar
• Format: Article
• Language: English
• ISSN: 1520-5827
• DOI: 10.1021/acs.langmuir.7b01408

Bio-active nanomaterials, namely: ruthenium hydrous oxide (or ruthenium oxy-hydroxide), RuOx(OH)y and also a surface-conjugated novel material of the same within the template of an amino acid molecule: L-cysteine have been studied. These compounds have been prepared through a simple wet chemical route, under physiological condition, such that they could be suitably used in anti-cancer applications. Several physical methods were used for the nano-material characterization, e.g.: thermal analysis of the as prepared ruthenium hydrous oxide by differential scanning calorimetry (DSC) followed by thermal gravimetric analysis (TGA). This confirms the material to be a precursor for anhydrous nano-crystalline ruthenium oxide (RuO2), as is affirmed by powder X-ray diffraction pattern. Also, optical spectroscopic absorption (UV-Vis and FT-IR) study of these nano-particles(NPs) to ascertain their surface conjugation with L-cysteine have been performed. Besides these, surface morphology of the NPs were studied by field emission scanning electron microscopy (FE-SEM) along with their elemental purity check through energy dispersive X-ray analysis (EDX). Their surface chemical micro-environments were examined by X-ray photo electron spectroscopy (XPS). The hydrodynamic size of the prepared NPs were measured through dynamic light scattering (DLS) studies. Further, biological consequences of these NPs on cancerous HeLa cells and their cytotoxicity effects have been reported with MTT assay, such an application has not been reported so far.