Simultaneous determination of ten kinds of biogenic amines in rat plasma using high-performance liquid chromatography coupled with fluorescence detection

• Published in: Biomedical chromatography 2018-02
• Main Authors: Wang, Xinna, Liang, Yawei, Wang, Yaqi, Fan, Mingqin, Sun, Yanni, Liu, Jianli, Zhang, Ning
• Format: Article
• Language: English
• ISSN: 1099-0801

We described a simple, rapid, selective and sensitive HPLC method coupled with fluorescence detection (HPLC-FLD) for simultaneous determination of ten kinds of biogenic amines (BAs: tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, 5-hydroxytryptamine, tyramine, spermidine, dopamine, spermine). BAs and I.S were derivated with dansyl chloride. A fluorescence detection (λex/λem = 340/510 nm) was used. A satisfactory result of method validation was obtained. The assay was shown to be linear over the range of 0.005-1.0 μg/mL for tryptamine, 2-phenethylamine and spermidine, 0.025-1.0 μg/mL for putrescine, 0.001-1.0 μg/mL for cadaverine, 0.25-20 μg/mL for histamine, 0.25-10 μg/mL for 5-hydroxytryptamine and dopamine, and 0.01-1.0 μg/mL for tyramine and spermine. The limits of detection (LODs) and the limits of quantification (LOQs) were 0.3-75.0 ng/mL and 1.0-250.0 ng/mL, respectively. Relative standard deviations (RSDs) were ≤ 5.14% for the intra-day and ≤ 6.58% for inter-day precision. The recoveries of BAs ranged from 79.11% to 114.26% after spiking standard solution of BAs to a sample at three levels. The seven kinds of BAs were found in rat plasma, and the mean values of tryptamine, 2-phenethylamine, putrescine, cadaverine, histamine, spermidine and spermine determined were 52.72±7.34 ng/mL, 11.45±1.56 ng/mL, 162.56±6.26 ng/mL, 312.75±18.11 ng/mL, 1306.50±116.16 ng/mL, 273.89±26.41 ng/mL, 41.51±2.07ng/mL, respectively.