Improving the stability of 11 C-labeled L-methionine with ascorbate

Carbon-11 labeled L-methionine ( C-MET) is a popular tracer used in the clinic for imaging brain tumors with positron emission tomography. However, the stability of C-MET in its final formulation is not well documented in literature. Recently, we observed fast degradation of HPLC-purified C-MET over...

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Bibliographic Details
Published in:EJNMMI radiopharmacy and chemistry 2017, Vol.2 (1), p.13
Main Authors: Woods, Michael, Leung, Leo, Frantzen, Kari, Garrick, Jennifer G, Zhang, Zhengxing, Zhang, Chengcheng, English, Wade, Wilson, Don, Bénard, François, Lin, Kuo-Shyan
Format: Article
Language:English
Online Access:Get full text
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Summary:Carbon-11 labeled L-methionine ( C-MET) is a popular tracer used in the clinic for imaging brain tumors with positron emission tomography. However, the stability of C-MET in its final formulation is not well documented in literature. Recently, we observed fast degradation of HPLC-purified C-MET over time, and systematic investigation was conducted to identify the cause. In this study, we verified the degraded product as C-labeled methionine sulfoxide ( C-METSO). To minimize oxidation, ascorbate (100 ppm) was added to the HPLC eluant, and the resulting HPLC-purified C-MET was stable in the final formulation solution without noticeable degradation for up to 1 h after the end of synthesis. Our data suggest that to minimize degradation, ascorbate can be added to the C-MET formulation solution especially if it is not administered into patients soon after the end of synthesis.
ISSN:2365-421X
DOI:10.1186/s41181-017-0032-x