Transcriptomic and Ectoine Analysis of Halotolerant Nocardiopsis gilva YIM 90087 T Under Salt Stress

The genus is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by YIM 90087 under salt stress. The colony color of YIM 90087 changed from yellow to...

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Bibliographic Details
Published in:Frontiers in microbiology 2018, Vol.9, p.618
Main Authors: Han, Jian, Gao, Quan-Xiu, Zhang, Yong-Guang, Li, Li, Mohamad, Osama A A, Rao, Manik Prabhu Narsing, Xiao, Min, Hozzein, Wael N, Alkhalifah, Dalal H M, Tao, Yong, Li, Wen-Jun
Format: Article
Language:English
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Summary:The genus is an unique actinobacterial group that widely distributed in hypersaline environments. In this study, we investigated the growth conditions, transcriptome analysis, production and accumulation of ectoine by YIM 90087 under salt stress. The colony color of YIM 90087 changed from yellow to white under salt stress conditions. Accumulation of ectoine and hydroxyectoine in cells was an efficient way to regulate osmotic pressure. The ectoine synthesis was studied by transferring the related genes ( , and ) to . Transcriptomic analysis showed that the pathways of ABC transporters (ko02010) and glycine, serine, and threonine metabolism (ko00260) played a vital role under salt stress environment. The from YIM 90087 was activated under the salt stress. Addition of exogenous ectoine and hydroxyectoine were helpful to protect YIM 90087 from salt stress.
ISSN:1664-302X
1664-302X