Magnetic nanoparticle decorated graphene based electrochemical nanobiosensor for H 2 O 2 sensing using HRP

To utilize synergetic effect of graphene's higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for H O sensing. Sensors based on magnetic nano...

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Bibliographic Details
Published in:Colloids and surfaces, B, Biointerfaces B, Biointerfaces, 2018-04, Vol.167, p.425
Main Authors: Waifalkar, P P, Chougale, A D, Kollu, P, Patil, P S, Patil, P B
Format: Article
Language:English
Online Access:Get full text
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Summary:To utilize synergetic effect of graphene's higher conductivity and magnetic nanoparticles biocompatibility, an electrochemical nanobiosensor is constructed based on magnetic nanoparticle decorated graphene (MRGO) using Horseradish peroxidase (HRP) for H O sensing. Sensors based on magnetic nanoparticles (MNP) and reduced graphene oxide (RGO) are studied for comparison. MNP, RGO and MRGO were characterized by X-ray diffraction (XRD), Transmission electron microscopy (TEM) and Fourier transform infrared spectroscopy (FTIR). XRD studies have confirmed successful synthesis of Fe O MNPs, RGO and MRGO. TEM micrographs revealed uniform decoration of MNPs on graphene. FTIR confirmed the immobilization of HRP on MNP, RGO and MRGO. The MRGO based sensor exhibited higher sensitivity (48.08 μA μM  cm ) compared to MNP (39.08 μA μM  cm ) and RGO (41.08 μA μM  cm ) based biosensors.
ISSN:1873-4367