Osteoinducing scaffolds with multi-layered biointerface

• Published in: Biomedical materials (Bristol) 2018-06, Vol.13 (5), p.054103-054103
• Main Authors: Grebenik, E A, Grinchenko, V D, Churbanov, S N, Minaev, N V, Shavkuta, B S, Melnikov, P A, Butnaru, D V, Rochev, Yu A, Bagratashvili, V N, Timashev, P S
• Format: Article
• Language: English
• Subjects: 3T3 Cells; Animals; Biocompatible Materials - chemistry; Bone Marrow Cells - cytology; Bone Substitutes - chemistry; Calcium - chemistry; Cell Differentiation - drug effects; Collagen - chemistry; Culture Media; Fibroblasts - cytology; Humans; Hyaluronic Acid - chemistry; Mesenchymal Stem Cells - cytology; mesenchymal stromal cells; Mice; Microspheres; multi-layered cell sheets; Osteoblasts - cytology; Osteogenesis - drug effects; osteoinduction; Polyesters - chemistry; polylactide; Signal Transduction; surface-selective laser sintering; Tissue Engineering - methods; Tissue Scaffolds - chemistry; tissue-engineering
• ISSN: 1748-605X; 1748-605X
• DOI: 10.1088/1748-605X/aac4cb

This study was aimed to design and characterise hybrid tissue-engineered constructs composed of osteoinducing polylactide-based scaffolds with multi-layered cellular biointerface for bone tissue reconstruction. Three-dimensional scaffolds with improved hydrophilic and osteoinducing properties were produced using the surface-selective laser sintering (SSLS) method. The designed scaffold pattern had dimensions of 8 × 8 × 2.5 mm and ladder-like pores (∼700 m in width). Hyaluronic acid-coated polylactide microparticles (∼100 m in diameter) were used as building blocks and water was used as the photosensitizer for SSLS followed by photocross-linking with Irgacure 2959 photoinitiator. Resulting scaffolds provided successful adhesion and expansion of human bone marrow mesenchymal stromal cells from a single-cell suspension. Induced calcium deposition by the cells associated with osteogenic differentiation was detected in 7-21 days of culturing in basal medium. The values were up to 60% higher on scaffolds produced at a higher prototyping speed under the experimental conditions. Innovative approach to graft the scaffolds with multi-layered cell sheets was proposed aiming to facilitate host tissue-implant integration. The sheets of murine MS-5 stromal cell line exhibited contiguous morphology and high viability in a modelled construct. Thus, the SSLS method proved to be effective in designing osteoinducing scaffolds suitable for the delivery of cell sheets.