Low-cost high sensitivity pulsed endomicroscopy to visualize tricolor optical signatures

A highly sensitive, modular three-color fluorescence endomicroscopy imaging platform spanning the visible to near-infrared (NIR) range is demonstrated. Light-emitting diodes (LEDs) were sequentially pulsed along with the camera acquisition to provide up to 20 frames per second (fps) three-color imag...

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Bibliographic Details
Published in:Journal of biomedical optics 2018-07, Vol.23 (7), p.076005-076005
Main Authors: Krstajić, Nikola, Mills, Bethany, Murray, Ian, Marshall, Adam, Norberg, Dominic, Craven, Thomas H, Emanuel, Philip, Choudhary, Tushar R, Williams, Gareth O. S, Scholefield, Emma, Akram, Ahsan R, Davie, Andrew, Hirani, Nik, Bruce, Annya, Moore, Anne, Bradley, Mark, Dhaliwal, Kevin
Format: Article
Language:English
Subjects:
Aged
Animals
Bronchoscopy
Clinical Trials as Topic
Endoscopy - economics
Endoscopy - instrumentation
Endoscopy - methods
Equipment Design
Female
Humans
Image Processing, Computer-Assisted
Limit of Detection
Lung - diagnostic imaging
Male
Microscopy, Fluorescence - economics
Microscopy, Fluorescence - instrumentation
Microscopy, Fluorescence - methods
Middle Aged
Molecular Imaging - economics
Molecular Imaging - instrumentation
Molecular Imaging - methods
Sheep
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Summary:A highly sensitive, modular three-color fluorescence endomicroscopy imaging platform spanning the visible to near-infrared (NIR) range is demonstrated. Light-emitting diodes (LEDs) were sequentially pulsed along with the camera acquisition to provide up to 20 frames per second (fps) three-color imaging performance or 60 fps single color imaging. The system was characterized for bacterial and cellular molecular imaging in ex vivo human lung tissue and for bacterial and indocyanine green imaging in ex vivo perfused sheep lungs. A practical method to reduce background tissue autofluorescence is also proposed. The platform was clinically translated into six patients with pulmonary disease to delineate healthy, cancerous, and fibrotic tissue autofluorescent structures. The instrument is the most broadband clinical endomicroscopy system developed to date (covering visible to the NIR, 500 to 900 nm) and demonstrates significant potential for future clinical utility due to its low cost and modular capability to suit a wide variety of molecular imaging applications.
ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.23.7.076005