iTRAQ-based quantitative proteomic analysis of differentially expressed proteins in chemoresistant nasopharyngeal carcinoma

Nasopharyngeal carcinoma (NPC) is a highly prevalent disease in Southeast Asia. The disease is typically diagnosed in the later stages, and chemotherapy resistance often causes treatment failure. To investigate the underlying mechanisms of drug resistance, we searched for chemoresistant-associated p...

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Bibliographic Details
Published in:Cancer biology & therapy 2018-09, Vol.19 (9), p.809-824
Main Authors: Wang, Kun, Chen, Zhen, Long, Lu, Tao, Ya, Wu, Qiong, Xiang, Manlin, Liang, Yunlai, Xie, Xulin, Jiang, Yuan, Xiao, Zhiqiang, Yan, Yahui, Qiu, Shiyang, Yi, Bin
Format: Article
Language:English
Subjects:
ANXA1
bioinformatics
chemoresistance
iTRAQ
nasopharyngeal carcinoma
proteomic profiles
Research Paper
TRIM29
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Summary:Nasopharyngeal carcinoma (NPC) is a highly prevalent disease in Southeast Asia. The disease is typically diagnosed in the later stages, and chemotherapy resistance often causes treatment failure. To investigate the underlying mechanisms of drug resistance, we searched for chemoresistant-associated proteins in NPC and drug-resistant NPC cell lines using isobaric tags for relative and absolute quantitation combined with nano liquid chromatography-tandem mass spectrometry. The chemoresistant NPC cell lines CNE1DDP and CNE2DDP were resistant to 1 mg/L cisplatin, had resistant indexes of 4.58 and 2.63, respectively, and clearly grew more slowly than the NPC cell lines CNE1 and CNE2. Using three technical replicates, we identified 690 nonredundant proteins, 56 of which were differentially expressed in both groups of cell lines (CNE1 vs. CNE1DDP and CNE2 vs. CNE2DDP). Gene Ontology, KEGG pathway, and miRNA analyses and protein-protein interactions of differentially expressed proteins showed that proteins TRIM29, HSPB1, CLIC1, ANXA1, and STMN1, among others, may play a role in the mechanisms of chemoresistance in clinical therapy. The chemotherapy-resistant proteomic profiles obtained may allow the identification of novel biomarkers for early detection of chemoresistance in NPC and other cancers.
ISSN:1538-4047
1555-8576
DOI:10.1080/15384047.2018.1472192